component-resolved  diagnosis (crd) using microarray technology has recently been introduced  with the  aim to  improve diagnosis of allergy. the  aim of this study was to compare performance of this allergen microarray to those of an established extract-based skin prick testing (spt). 45  patients  with  allergic rhinitis  were studied  (16 children  and  29  adults). spt  to dermatophagoides ...

Household arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach, Periplaneta fuliginosa. Enzyme-linked immunosorbent assay...

C-reactive protein and four other acute phase reactant proteins of non-cardiac, origin (orosomucoid, alpha 1- antitrypsin, heptoglobin, and alpha 2- macroglobulin) were studied serially by laser immunonephelometric assay in sera from 17 patients with myocardial infarction. A similar comparison was made in 57 patients undergoing surgery and 72 patients with acute infection. C-reactive protein wa...

BACKGROUND Seawater and freshwater shrimp are some of the most common causes of food allergy among children in Thailand. Tropomyosin has been reported as a major allergen for shrimp allergic populations around the world. Despite a high number of shrimp-allergic Thai children, however, it is unknown whether shrimp tropomyosin is a major cause of allergic reactions. OBJECTIVES To clone and char...

Background Allergenic tropomyosins are highly cross-reactive panallergens found in various invertebrates. Sensitized patients can develop allergic responses ranging from oral allergy syndrome to severe anaphylactic reactions after the ingestion of tropomyosin-containing food. The usual routs of sensitization to tropomyosin include the airborne exposure to various mites and cockroaches and the o...

OBJECTIVES We investigated the incidence and contribution of the oxidation/nitrosylation of tropomyosin and actin to the contractile impairment and cardiomyocyte injury occurring in human end-stage heart failure (HF) as compared with nonfailing donor hearts. BACKGROUND Although there is growing evidence that augmented intracellular accumulation of reactive oxygen/nitrogen species may play a k...

During muscle contraction, a molecular interaction takes place between the myofilament proteins actin and myosin, which is triggered by a rise in intracellular calcium and is driven by the energy from ATP hydrolysis. The tropomyosin– troponin complex inhibits the actin–myosin interaction at low intracellular-free calcium. This inhibition is released when intracellular-free calcium increases and...

N(ε)-(Hexanoyl)lysine, formed by the reaction of lysine with n-6 lipid hydroperoxide, is a lipid peroxidation marker during the initial stage of oxidative stress. The aim of the present study is to indentify N(ε)-(hexanoyl)lysine-modified proteins in neoplastic transformed gastric mucosal cells by N-methyl-N'-nitro-N-nitrosoguanidine, and to compare the levels of these proteins between gastric ...

Since tropomyosin is cross reactive in many arthropods, it was assumed that this highly conserved protein could be responsible for cross reactions in house dust mite (HDM) allergic patients who experienced adverse reactions after crustacean and mollusc ingestion. Here we report two clinical cases where the role of tropomyosin is a matter of debate. In the first case, the clinical history, as we...

Two muscle differentiation programs, acetylcholinesterase and tropomyosin-containing filaments and fibrils, occur together in the same cleavage-arrested zygotes (1-celled) of the ascidian Ciona intestinalis. Coexpression in such undivided but developing 'embryos' is consistent with the idea that separate elements of muscle differentiation are related at some regulatory level, perhaps through a ...