Aim: To assess whether the use of direct realtime polymerase chain reaction (PCR) on smear-positive sputa can be cost-effective, by speciating mycobacteria earlier than current methods and thereby preventing unnecessary screening tests as part of the contact tracing process. Methods: A retrospective study of all patients with smear-positive sputa in a Liverpool teaching hospital between 2004 an...

Abstract Full scale simulations of neuronal network models the brain are challenging due to high density connections between neurons. This contribution reports run times shorter than simulated span biological time for a full model local cortical microcircuit with explicit representation synapses on recent conventional compute node. Realtime performance is relevant robotics and closed-loop appli...

It is challenging to find genes with stable transcripts for use as reference genes for quantitative realtime polymerase chain reaction (qRT-PCR) during viral infection. Autographa californica nucleopolyhedrovirus (AcMNPV) is known to globally shut off host gene transcription in Sf21 cells and to modify their cytoskeletons. In this study, seven host genes were selected for validation as referenc...

Bovine viral diarrhea virus (BVDV) and bovine rotavirus (BRV) are important pathogens of cattle. These pathogenselicitsigns that is sometimes difficult to differentiate. A real-time duplex RT-PCR assay would allow the simultaneous detection of the two viruses in the same sample. A real-time duplex RT-PCR assay was developed and optimized. The detection limit of the assay was 5 TCID50 or 100 RNA...

A novel real-time PCR assay was developed for the direct detection in food of Helicobacter pullorum-like bacteria, which are occasionally associated with human enteric disease. Experiments using control strains showed that the realtime PCR assay was specific and reproducible, with a detection level of 1 colony-forming unit (CFU)/g. The assay was then applied to determine contamination rates in ...

Researchers carrying out quantitative, multiplex, realtime PCR and RT-PCR require master mixes that provide accurate, simultaneous quantification of multiple targets in the same reaction without time-consuming optimization. Our results at Wyeth Research show that QuantiTect® Multiplex Kits from QIAGEN are well suited for multiplex analysis of up to four RNA targets in two-step and onestep real-...

Pembersihan bak sampah yang dilakukan petugas kebersihan terkadang mengalami keterlambatan karena pemerikasaan secara manual. Perihal ini mengurangi performa dalam pembersihan di titik berbeda. Maka salah satu solusinya adalah menciptakan sebuah sistem monitoring realtime. Sistem tersebut berbasis Internet of Things dan Android sudah terintegrasikan dengan Firebase Realtime Database. memakai se...

Interleukin-36 has been implicated in the pathogenesis of various skin disorders. We previously reported that Il36rn-/- mice revealed delayed wound healing due to excessive recruitment immune cells. In this study, we examined effect dimethyl fumarate (DMF), which is an modulator and inducer antioxidant response, for mice. Full-thickness excisional wounds were produced on back wild was assessed ...