In children with severe bacterial infection in low-resource settings, switch from intravenous to oral antibiotics is important reduce nosocomial infections and costs. We report barriers reliable antibiotic administration under five admitted Kisantu hospital (DR Congo) bloodstream infection. Qualitative observations were compiled during field studies (DeNTS/TreNTS study: NCT04473768 /04850677). ...

Hill activity (oxygen evolution with ferricyanide as the electron acceptor), light-induced absorbance changes at liquid nitrogen temperature associated with the primary activity of photosystem II, and fluorescence yield changes at both low temperature and room temperature were measured with lyophilized spinach chloroplasts before and after extraction with hexane and reconstitution with beta-car...

To paraphrase Oscar Wilde, one could say that clinical transplantation imitates mouse models. This has led to extensive knowledge of graft-versus-host disease (GVHD) pathophysiology, although it remains a serious complication after transplant. The pervasive nature of acute GVHD is becoming increasingly apparent, as the list of potential targets has expanded beyond the skin, liver, and gastroint...

Functional large ribosomal subunits of Thermus aquaticus can be reconstituted from ribosomal proteins and either natural or in vitro transcribed 23 S and 5 S rRNA. Omission of 5 S rRNA during subunit reconstitution results in dramatic decrease of the peptidyl transferase activity of the assembled subunits. However, the presence of some ribosome-targeted antibiotics of the macrolide, ketolide or...

The optimal approach for stem cell transplantation in children with immunodeficiency has yet to be determined. Conditioning therapy is necessary for reliable engraftment and full immune reconstitution; however, the beneficial effect of cytoreductive conditioning is counterbalanced by increased short- and long-term treatment-related toxicity. Whether bone marrow transplantation with a nonmyeloab...

Reconstitution of human cytomegalovirus (HCMV)–specific cytotoxic T lymphocytes (CTLs), predominantly directed against pp65, provides protective immunity for the development of HCMV disease after allogeneic stem cell transplantation (SCT). To define pp65-derived CTL epitopes that would allow sensitive detection of HCMV-specific immune reconstitution, a computer-based epitope prediction was perf...