background and objective : due to the evolution of multidrug-resistant strains, screening of natural resources, especially actinomycetes, for new therapeutic agents discovery has become the interests of researchers. in this study, molecular, chemical and biological screening of soil actinomycetes was carried out in order to search for peptide-producing actinomycetes. materials and methods : 60 ...

An increasing number of cyclopeptides have been discovered as products of ribosomal synthetic pathway. The biosynthetic study of these cyclopeptides has revealed interesting new mechanisms for cyclization. This review highlighted the recent discoveries in cyclization mechanisms for cyclopeptides synthesized independently of non-ribosomal peptide synthetases, including endopeptidase-catalyzed cy...

We have previously proposed that the BlmIV and BlmIII non-ribosomal peptide synthetases are involved in the formation of the bithiazole moiety of the anti-tumor drug bleomycin in Streptomyces verticillus ATCC15003. We report here the identification and characterization of an oxidation domain in BlmIII. The oxidation domain shows local homology to a family of oxidoreductases and is present in al...

Abstract Non-ribosomal peptide synthetases are important enzymes for the assembly of complex natural products. Within these multi-modular lines, condensation domains perform central function chain assembly, typically by forming a bond between two peptidyl carrier protein (PCP)-bound substrates. In this work, we report structural snapshots domain in with an aminoacyl-PCP acceptor substrate. Thes...

ClusterMine360 (http://www.clustermine360.ca/) is a database of microbial polyketide and non-ribosomal peptide gene clusters. It takes advantage of crowd-sourcing by allowing members of the community to make contributions while automation is used to help achieve high data consistency and quality. The database currently has >200 gene clusters from >185 compound families. It also features a uniqu...

Cyclodipeptides are diverse chemical scaffolds that show a broad range of bioactivities relevant for medicine, agriculture, catalysis, and material sciences. can be synthesized enzymatically through two unrelated enzyme families, non-ribosomal peptide synthetases (NRPS) cyclodipeptide synthases (CDPSs). The diversity cyclodipeptides is derived from the amino acid side chains modification those ...