rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...

Background and Aims: Hepatitis C virus is one of the viral infections which is mainly transmitted by blood transfusion. Patients with thalassaemia frequently need blood transfusion and are in danger of HCV infection. In most cases of infection (85%) the virus evades the immune system and establishes a chronic infection that may lead to cirrhosis and liver carcinoma. Liver is the main site of HC...

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...

Die RNA ist ein Molekül, das sowohl genetische Informationen speichern als auch katalytische Reaktionen ausführen kann. Dieser beobachtete Dualismus rückt die ins Rampenlicht der Konzepte über den Ursprung des Lebens. RNA-Welt-Hypothese geht davon aus, dass Leben aus selbst replizierenden RNA-Molekülen entstand, sich zu immer komplexeren Strukturen entwickelten. Vor kurzem haben wir gezeigt, mi...

Noncoding ribonucleic acids micro-RNA is involved in the regulation of gene expression have major roles in the post-transcriptional level. A micro-RNA alone several causes down regulation of mRNA transcript of the target. Thus, small changes in the expression of a micro RNA may lead to significant changes in gene expression are different. Micro- RNA as key regulators of immune cell lineage diff...

Background: The stress is one of main factors effects on production system. Several factors (both genetic and environmental elements) regulate immune response to stress. Objectives: In order to determine the major immune system regulatory genes underlying stress responses, a learning Bayesian network approach for those regulatory genes was applied to RNA-...

lots of viruses, in particular rna viruses, have high mutation rates and relatively short generation times. particle stability during infection in nature or in laboratory triggers the evolutionary event toward different mechanisms such as genome segmentation, point mutation and  recombination. the frequency of mutant genomes increase and modify  the previous distribution, which, consequently, l...