conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

Real-time RT-PCR is used as a gold standard method for the diagnosis of COVID-19. Since real-time RT PCR nucleic acid-based, it highly sensitive and specific test. However, this test takes 4–8 h to generate results and, in emergency settings, delay may prove fatal certain patients. The frequent surge COVID cases increases patient load settings. Thus, acid-based rapid POC required that can quick...

abstract beet yellows virus (byv), the type member of the genus closterovirus and one of the most economically important yellowing viruses, causes serious losses in sugarbeet. in order to study infection and distribution of beet yellows virus during summer 2007, about 530 samples were collected from beet’s fields in razavi khorasan province (fariman, torbate jam, ghoochan, chenaran, torbate hey...

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

asiatic citrus canker is a devastating disease resulting in drastic economic losses in citriculture worldwide. amongst three different types of the disease, i.e. a, a* and aw, the a* type is genetically less known. in order to comprehend the behavior of the asiatic citrus canker a*-type strain (xanthomonas citri subsp. citri) in the vicinity of the host cells, a targeted semi-quantitative trans...