somatic embryogenesis is a new approache to plant mass in vitro propagation. this method influences by elements and their components in medium. in order to investigate the effects of different media and it,s role on somatic embryogenesis of carrot, a cbd experiment with four replications (including 12 treatments) was conducted. to do this, carrot petioles (with 1 cm dimension) in induction phas...

salicylic acid play an important role in plants physiological processes. in this research the effect of salicylic acid on induction of somatic embryogenesis and plant pigments synthesis in the phloem and petiole explants of carrot in the b5 medium was investigated. we added treatments with 0, 0.5, 1.5, 2 and 3 mm to culture medium by experimental base on the randomized design to investigate the...

The present study was conducted in two different experiments based on complete randomized design to evaluate the effects of calcium (0, 1, 2, 4 mM) and magnesium (0, 1.02., 2.04, 4.08 mM) on carrot petiole somatic embryogenesis in B5 medium. A difference on somatic embryogenesis was observed among different levels of calcium and magnesium. The number of globular embryos in medium with low magne...

one of the important methods of plant propagation in large scale so as genetic manipulation and artificial seed production is somatic embryogenesis technique. the obtained results from this methods related to different factors including condition of culture medium. therefore, the aim of this study conduction were determining the role of molybdenum during carrot petiole somatic embryogenesis. at...

Many factors may influence the efficiency of somatic embryogenesis. This capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. The effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mM) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

many factors may influence the efficiency of somatic embryogenesis. this capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. the effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mm) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

abstract somatic embryogenesis is a new approach to in vitro plant propagation. this technique is based on stem cell obtain and its differentiation to a complete plant. this is affected by components of media culture. so, the effects of ms, b5 and nl media on carrot petiole somatic embryogenesis investigated via histological experiment. to do this, carrot petioles (with 1 cm dimension) cultured...

bryogenesis is usefu1 for isolating genes expressed during embryogenesis, as embryegenic competence and the expressien of certain embryo-specific genes increases with increased stress. Fluorescent differential display (FDD) was perfonmed to isolate stress-induced genes in carrot somatic embryos, and five genes were isolated as embryonic tissue-specific genes. One ofthese genes was expressed unt...

The role of reduced and oxidized glutathione in plant development was investigated using wild carrot suspension cultures. Concentrations of GSH are lower in developing than in proliferating carrot cultures. Addition of 0.3 mM-buthionine sulfoximine (a glutathione synthesis inhibitor) to developing cultures decreased the cellular GSH levels and enhanced somatic embryogenesis while addition of 0....