This study compared SYBR Green real-time quantitative PCR (qPCR) with standard plate counting for the enumeration of Streptococcus mutans in oral samples. Oral samples (n = 710) were collected from high-caries-risk children for quantification of S. mutans by qPCR using primer pairs. The S. mutans copy number was calculated with reference to a qPCR quantification cycle (Cq) standard curve and co...

A sequence of 1647 base pairs in length of Streptococcus mutans DNA that encodes for a 63-kDa protein with significant amino acid similarity with fibronectin-binding proteins of Streptococcus pyogenes and Streptococcus gordonii was cloned. The putative recombinant fibronectin-binding protein of S. mutans was purified using affinity chromatography and the cloned protein was used to prepare polyc...

Streptococcus mutans has been recognized as an important etiological agent in human dental caries. Some strains of S. mutans also produce bacteriocins. In this study, we sought to demonstrate that bacteriocin production by S. mutans strains GS5 and BM71 was mediated by quorum sensing, which is dependent on a competence-stimulating peptide (CSP) signaling system encoded by the com genes. We also...

OBJECTIVE This study aims to elucidate the effect of IgY anti ComD on the biological properties of Streptococcus mutans. (S. mutans) ComD is an interspecies quorum-sensing signaling receptor that plays an important role in biofilm formation by S. mutans. MATERIALS AND METHODOLOGY Egg yolk IgY was produced by the immunization of chickens with a DNA vaccine containing the ComD DNA coding region...

Background and Objective: Systemic sclerosis is an autoimmune disorder with orofacial manifestations, including tooth decay. Lactobacilli can inhibit biofilm formation and growth of cariogenic pathogens, such as Streptococcus mutans. We aimed to assess the salivary levels of S. mutans and Lactobacilli as indicators of dental caries development in patients with systemic sclerosis. Methods: In t...

background: orthodontic therapy has oral ecological changes causing increased numbers of mutans streptococci in saliva and plaque. the purpose of this study was to estimate counts and colonization pattern of streptococcus mutans after application of fixed orthodontic appliances. materials and methods: plaque samples of randomly selected sixty patients were collected before placement of orthodon...

background:dental caries is associated with oral pathogenes and streptococcus mutans (s. mutans)is one of the primary cariogenic organisms. the aim of this clinical study was to evaluate the effect of sugar free chewing gum containing casein phosphopeptide-amorphous calcium phosphate(cpp-acp) and xylitol on salivary streptococcus mutan.   m aterials and methods: 60 dental students, who voluntee...

Background and Aim: This study aimed to compare the effects of acetone and ethanolic extracts of Plantago major (P. major) on S. mutans.   Materials and Methods: In this in vitro study, the antibacterial effects of eight different concentrations of the acetone and ethanolic extracts of P. major seed were examined on S. mutans, and the minimum inhibitory concentration (MIC) and minimum bacte...

BACKGROUND Streptococcus mutans and Streptococcus sobrinus are considered to be important bacterial species in the initiation of human dental caries. Therefore, the establishment of a reliable genotyping method to distinguish S. mutans from S. sobrinus is of central importance. OBJECTIVE We assessed the usefulness of repetitive extragenic palindromic polymerase chain reaction (rep-PCR) using ...