Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commer...

CONTEXT AND OBJECTIVE The molecular characterization of local isolates of Toxoplasma gondii is considered significant so as to assess the homologous variations between the different loci of various strains of parasites. DESIGN AND SETTING The present communication deals with the molecular cloning and sequence analysis of the 1158 bp entire open reading frame (ORF) of surface antigen 3 (SAG3) ...

PURPOSE Surface antigen 3 (SAG3) of Toxoplasma gondii is very similar in structure to the major surface antigen 1 (SAG1). Although numerous studies have supported the importance of SAG1 in protection against T. gondii infection, few reports exist on SAG3. MATERIALS AND METHODS Glutathione-S-transferase (GST)-fused SAG3 of T. gondii (rSAG3) were immunized into BALB/c mice alone or in combinati...

The current study included the detection of Toxoplasma gondii from aborted women Maternity and Childhood Teaching Hospital. It drew attention to human infection rate in order detect differentiation them with SAG3 gene. samples were collected during period October, 2021 until end March 2022 AL-Qadisyah province. Total 100 (placenta fetus) send laboratory veterinary medicine AL- Qadisiyah Univers...

BACKGROUND The coccidian protozoa Toxoplasma gondii is an obligate intracellular parasite of humans and other warm-blooded animals. Diagnosis of toxoplasmosis is of considerable medical importance for human, especially pregnant women and immunocompromised individuals. The apply of an Escherichia coli recombinant antigen(s) would be significantly useful in developing standardization of the diagn...

background: the coccidian protozoa toxoplasma gondii is an obligate intracellular parasite of humans and other warm-blooded animals. diagnosis of toxoplasmosis is of considerable medical importance for human, especially pregnant women and immunocompromised individuals. the apply of an escherichia coli recombinant antigen(s) would be significantly useful in developing standardization of the diag...

Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diap...

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10....

The study aimed to investigate the differences in genetic tree of types infected (Free living and Industrial chickens) with Toxoplasma parasite, (brain liver) collecting samples Al-Qadisiyah province during period from October 2021 until end March 2022. A total 90 chicken were collected, 60 brain 30 liver. purpose diagnosis, impression method was used. It found that free chickens all positive f...