Our objectives were to determine if milk casein as a percentage of true protein (CN%TP) estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is equivalent CN%TP Kjeldahl, and the proportion (CN), proteolysis products (CNPP), serum (SP) from (TP) that goes into Kjeldahl noncasein nitrogen (NCN) filtrate stays in NCN precipitate using SDS-PAGE. Raw samples collected 1...

An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein...

Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered samples received increasing concentrations (0, 1, 2.5, 5, 10, 20, 30, 50, 100% v/v), were subjected electrophoresis ...

The combination of SDS-PAGE and MS is one of the most powerful and perhaps most frequently used gel-based proteomics approaches in protein identification. However, one drawback of this method is that separation takes place under denaturing and reducing (R) conditions and as a consequence, all proteins with identical apparent molecular mass (Mr) will run together. Therefore, low-abundant protein...

We report that subunits of human nuclear proteasomes carry a previously unrecognised, constitutive posttranslational modification. Subunits with this modification are not visualised by SDS-PAGE, which is used in almost all denaturing protein gel electrophoresis. In contrast, CTAB-PAGE readily visualises such modified subunits. Thus, under most experimental conditions, with identical samples, SD...

conclusions the overall results indicated that bacteriophages isolated from sewage exhibited excellent lytic activity against mrsa strains. in conclusion, bacteriophages can be further characterized and appear to be a promising candidate for phage therapy against mrsa in the future. results the phages exhibited good lytic activity against mrsa when tested in-vitro, and the highest activity was ...

conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...