enrofloxacin is a synthetic chemotherapeutic agent from the class of the fluoroquinolones that is widely used to treat bacterial infections. it is metabolized to ciprofloxacin in the body as active metabolite. fluoroquinolones change in the articular cartilage, especially with high doses and more than two weeks use. so, due to relatively excessive use of enrofloxacin in mammals and similarity o...

Chondrocytes' hypertrophy includes metabolic changes, matrix remodelling, proliferation and apoptosis, characteristics associated with the progression of osteoarthritis. We investigated a possible association among Runt-related transcription factor 2 (RUNX-2), SOX-9 and fibroblast growth factor (FGF)-23 mRNA expressions in articular chondrocytes in order to elucidate their contribution in the o...

Tight junctions have recently emerged as essential signaling regulators of proliferation and differentiation in epithelial tissues. Here, we aimed to identify the factors regulating claudin-7 expression in the colon, and analyzed the consequences of claudin-7 overexpression in colorectal carcinoma (CRC). In healthy human colonic crypts, claudin-7 expression was found to be low in the stem/proge...

The aim of this study was to explore the mechanisms of Hyperbaric oxygen (HBO) protective on interleukin-1β (IL-1β) induced rat's mandibular condylar chondrocytes. Chondrocytes were exposure to Hyperbaric oxygen after induced inflammatory by IL-1β. After that, the expression of p-JNK and c-Jun was increased significantly, while the Sox-9 was decreased significantly, Immunofluorescence results s...

Introduction: Anabolic activity is a crucial activity in articular chondrocytes and its failure is one major reason of osteoarthritic cartilage degeneration. Unclear are the intracellular factors responsible for upand down regulation of the molecules of interest. In this respect, the transcription factor Sox-9, a member of the HMG-domain transcription factor family, elicited much of interest as...

Thimios A. Mitsiadis1,2, Marie-Laurence Mucchielli1, Expression of the Sylva Raffo2, Jean-Pierre Proust2, Peter Koopman3 and Christo Goridis1 transcription factors Otlx2, 1Laboratoire de Génétique et Physiologie du Développement, IBDM, Campus de Luminy case 907, 2IMEB EA 2198, Faculté Barx1 and Sox9 during d’Odontologie, Université de la Méditerranée, Marseilles, France, and 3Centre for Molecul...

The expression patterns of the transcription factor, Sox-9, and of the nuclear factor, cDachsund-2, were examined in the developing chick telencephalon. Both genes were expressed in the ventricular zone and in the subventricular zone of the telencephalon during the period of neurogenesis. Whereas Sox-9 was not expressed in postmitotic tissues, cDachsund-2 was specifically expressed in the neost...

BACKGROUND Sox 9 is a major marker of chondrocyte differentiation. When chondrocytes are cultured in vitro they progressively de-differentiate and this is associated with a decline in Sox 9 expression. The active form of vitamin D, 1, 25 (OH)2D3 has been shown to be protective of cartilage in both humans and animals. In this study equine articular chondrocytes were grown in culture and the effe...

To assess different effects of royal Jelly in protecting the human endothelial cells from high glucose level, human umbilical vein endothelial cells were exposed to various concentrations of royal jelly, from 0.625 to 10 mg/ml, at the presence of 5 and 30 mM glucose contents over a course of 72 h. In addition to cell viability assessment by conventional MTT assay, we also analyzed the feature o...

The Y chromosomal switch gene SRY, which initiates testis development during mammalian embryogenesis was recently described (l).The SRY-encoded protein contains a 79 amino acid DNA-binding motif referred to as an HMG-box (2) known to exist in other transcription factors or proteins involved in DNA bending (3). On the basis of the homology between their HMGboxes, other SRY-related genes have bee...