The Soil Plant Analysis Development (SPAD) chlorophyll meter is one of the most commonly used diagnostic tools to measure crop nitrogen status. However, the measurement method of the meter could significantly affect the accuracy of the final estimation. Thus, this research was undertaken to develop a new methodology to optimize SPAD meter measurements in rice (Oryza sativa L.). A flatbed color ...

The estimation of nitrogen status, non-destructively, in oilseed rape was performed using spectral reflectance with visible and near infrared reflectance spectroscopy (Vis/NIRS), and SPAD values of the oilseed rape leaves of 30 plots were measured by a SPAD 502 chlorophyll meter, and the research was carried out at experiment field in the Zhejiang University during growing season from 2007 to 2...

The techniques utilized to extract chlorophyll from plant materials are destructive and based on methods that use organic solvents. This study proposes the non-destructive quantification of chlorophyll and nitrogen concentration using a portable chlorophyll meter (SPAD-502). Further, was possible to establish relationships between chlorophyll fluorescence and SPAD-502 values. This methodology c...

monly used instruments is a Minolta SPAD (soil plant analysis development) meter. The SPAD meter measures the difference in light absorption at 430 and 750 nm (Wood et al., 1992). The former is the transmittance peak for chlorophyl ‘a’ and ‘b’, and the latter is in the near-infrared region. The instrument converts the difference in light absorption at these two wavelengths into a numerical SPAD...

Nitrogen (N) management is critical in optimizing potato yield and quality and reducing environmental pollution. Six N rates from 34 to 270 kg ha , and different timing of N application were used in a 3-year field experiment to contrast SPAD-502 chlorophyll meter and QuickBird satellite imagery data against the conventional petiole sampling technique for assessing canopy N status. Overall treat...

Rice leaves display lateral asymmetry around the midrib, and the narrow side exhibits higher leaf area-based nitrogen concentration (Na) and soil plant analysis development (SPAD) values than the wider side. However, the difference in the relationship between the SPAD of each side and Na of the corresponding lateral half, and the optimal position along the leaf blade for SPAD measurements are n...

A Soil-Plant Analysis Development (SPAD) chlorophyll meter can be used as a simple tool for evaluating N concentration of the leaf and investigating the combined effects of nitrogen rate and leaf age on N distribution. We conducted experiments in a paddy field over two consecutive years (2008-2009) using rice plants treated with six different N application levels. N distribution pattern was det...

field experiment was conducted in 2008 at the research field of agricultural college of shiraz university, shiraz, iran to investigate the effects of urban waste compost (uwc), manure and chemical fertilizer on the initial growth of corn (zea mays). experimental design was split plot factorial with three replications. main plots were assigned to nitrogen (n) fertilizer (0 and 200 kg n ha-1), an...

Chlorophyll meters are widely used to guide nitrogen (N) management by monitoring leaf N status in agricultural systems, but the effects of environmental factors and leaf characteristics on leaf N estimations are still unclear. In the present study, we estimated the relationships among SPAD readings, chlorophyll content and leaf N content per leaf area for seven species grown in multiple enviro...

The quantum yield of photosystem II, determined by chlorophyll fluorescence and the quantum yield of CO2 uptake, determined from gas exchange, are two physiologically important measurements in clarifying the response to environmental stress. Both measurements, however, require an accurate assessment of leaf light absorption in the photosynthetically active radiation wavelength range (400–700 nm...