background: anti-sperm antibody (asa) can decrease sperm motility and, therefore, it is a cause of male infertility. the aim of this study was to evaluate the effects of varicocelectomy on anti-sperm antibody in patients with varicocele. methods: this observational study was conducted on 90 patients with varicocele at sina and imam khomeini hospitals during 2006 to 2009. all varicocelectomy can...

background: antifertility effect of naturally occuring antisperm antibody (asa) in infertile couples and studies on experimental immunization of various animals with sperm antigens represents asa as immunocontraceptive target. despite extensive research on the effects of different factors on sperm immunogenecity and asa production variable result have been reported. objective: to study whole sp...

introduction: antifertility effects of naturally occuring antisperm antibody (asa) in infertile couples and studies on experimental immunization of various animals with sperm antigens represent asa as an immunocontraceptive target. the effects of different factors on sperm immunogenecity and asa production have been studied and different results have been reported. in this study, whole sperm im...

BACKGROUND Contraceptive vaccines can provide valuable alternatives to current methods of contraception. We describe here the development of sperm-reactive human single chain variable fragment (scFv) antibodies of defined sperm specificity for immunocontraception. METHODS Peripheral blood leukocytes (PBL) from antisperm antibody-positive immunoinfertile and vasectomized men were activated wit...

BACKGROUND Sperm proteins are important for the sperm cell function in fertilization. Some of them are involved in the binding of sperm to the egg. We characterized the acrosomal sperm protein detected by a monoclonal antibody (MoAb) (Hs-8) that was prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperms and we tested the possible role of this protein in the bindi...

Among the numerous anti-sperm monoclonal antibodies generated in our laboratory, HS-33 was shown to react with a conserved antigen on the acrosome of spermatozoa from human and mouse. By using indirect immunofluorescent assay, it was demonstrated that HS-33 did not bind to live human sperm. However, this antibody was found to react with the methanol-fixed acrosome-intact, but not with acrosome-...

introduction: membrane cofactor protein (mcp or cd46) is one of the regulatory of complement system. it is present in all somatic cells except in rbc. it is also present in all body fluids and semen. the aim of this study was measuring the concentration of this protein in semen of fertile and infertile individuals and to study the relationship between the concentration of this protein and semen...

BACKGROUND Poor semen quality is one of the main causes of infertility. We have generated a set of monoclonal antibodies to human sperm and used them to investigate sperm quality. Some of these antibodies found differences in the expression of proteins between normal sperm and pathological sperm displaying severe defects. One of them was the Hs-14 antibody. The aim of this paper was to determin...

objective: catsper is a voltage-sensitive calcium channel that is specifically expressed in the testis and it has a significant role in sperm performance. catsper (1-4) ion channel subunit genes, causes sperm cell hyperactivation and male fertility. in this study, we have explored targeting of the extracellular loop as an approach for the generation of antibodies with the potential ability to b...

BACKGROUND Antibodies to human sperm are useful diagnostic reagents for detection of changes in sperm protein expression and their relationship with sperm defects and male infertility. The specificity of Hs-16 monoclonal antibody (mAb) and the localization and frequency of the occurrence of Hs-16-recognized protein on human spermatozoa were investigated. METHODS Samples from 30 fertile men wi...