introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

a simple special protocol using semichon’s acetic carmine stain was applied for examination of muscles of some small mammals, naturally infected with sarcocystis spp. using this method, infected muscles fibers with sarcocystis could easily be differentiated from muscle tissues. this method can be applied for screening of infected muscles with sarcocystis cysts on large number of samples for epi...

background: lymphadenitis is a common form of extra-pulmonary tuberculosis. as in peripheral health centers, the facili­ties for histopathology and culture are lacking, material obtained by fine needle aspiration (fna) in suspected cases of tubercu­lar lymphadenitis (tbl) cid fast bacilli (afb) by ziehl neelson method as every designated micros­copy centre under revised national tuberculosis co...

background : cd117 is a thyrosin kinase receptor encoded by c-kit proto-oncogene. it is expressed during normal development in some tissues and also in a subset of neoplasia especially gastrointestinal stromal tumors   (gists). treatment with thyrosin kinase inhibitors (e.g., imatinib) is useful in cd117- positive gists. the goal of this study is to investigate the expression of cd117 in glial ...

argyrophilic staining of nucleolar organizer regions (agnor) has been considered to be useful in the diagnosis and prognostic evaluation of different cutaneous tumors. in order to evaluate the role of the agnor technique in discriminating aggressive from non-aggressive basal cell carcinoma (bcc), paraffin-embedded histologic sections from 30 cases of aggressive bcc (bcc2) and 30 cases of non-ag...

nucleolar organizer regions (nor) are defined as nucleolar components containing a set of argyrophilic proteins which are selectively stained by colloidal silver nitrate staining. although studies have shown that the number of nor dots or particles is directly related to the rapidity of cell proliferation in cancer cells, prognostic or diagnostic value of nor remains controversial. the aim of t...

background: tuberculosis continues to be a serious public health problem causing nearly three million deaths per year all over the world. despite major improvement in diagnosis, it is not possible to control the disease in the absence of surveillance treatment, and follow-up programs. objectives: this research was designed to study the frequency of mycobacterium tuberculosis among specimens ref...

herpetosiphon giganteus is a filamentous gliding bacterium. gliding motility is the movement of the cells over surfaces without the aid of flagella. the mechanism responsible for bacterial gliding motility has not been known and there are only a few data on herpetosiphon giganteus. the aim of this study was to observe the ultrastructure and negative staining of isolated strains of herpetosiphon...

background and objective: tuberculosis is still a major health problem, involving about 1/3 of the world´s population. diagnosis is difficult when we only use ziehl-neelson staining. many cases may be missed. a more rapid and sensitive diagnostic method is necessary. pcr may be helpful. the aim of this study was to compare pcr, zieh-neelsen staining and histopathologic findings in diagnosis of ...