The immobilization of cross-linked tannase onto carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) was achieved via physical adsorption. Glutaraldehyde used to cross-link the enzyme molecules. Spectroscopic and morphological characterizations enzyme-nanotubes composite were carried out, which authenticated successful adsorption event. Enzyme is proven equal to, or even superior ...

VIII. Location of Tannase IX. Purification of Tannase X. Properties of Tannase A. pH Optimum and Stability B. Temperature Optimum and Stability C. Molecular Mass D. Enzyme Inhibition E. Kng of Tannase F. Carbohydrate Content XI. Isozymes of Tannase XI. Mode of Action of Tannase XIII. Immobilization of Tannase XIV. Applications of Tannase A. Instant Tea B. Beer Chillproofing C. Wine Making D. Pr...

BACKGROUND AND PURPOSE Microbial tannase (tannin acyl hydrolase, EC 3.1.1.20), a hydrolysable tannin-degrading enzyme, has gained importance in various industrial processes, and is used extensively in the manufacture of instant tea, beer, wine, and gallic acid. Tannase is an inducible enzyme, and hydrolysable tannin, especially tannic acid, is the sole inducer. This study is of the effect of va...

background: tannase can be obtained from the various sources for example tannin rich plants; however microbial sources are preferred for industrial production. in microbial sources, the aspergillus and penicillium genus and lactic acid bacteria mostly produce tannase. however, it has been identified that this enzyme is produced by many fungi and bacteria, but researches are continuing to find n...

Tannase (EC 3.1.1.20) is an enzyme that hydrolyzes the ester and depside bonds of tannic acid to gallic acid and glucose. In the production of foods and beverages, it contributes to the removal of the undesirable effects of tannins. The aim of this study is to investigate the potential of endophytic fungi isolated from jamun (Syzygium cumini (L.) Skeels) leaves, and identified as Pestalotiopsis...

A fungal tannase was produced, recovered, and immobilized by entrapment in calcium alginate beads. Catalytical properties of the immobilized enzyme were compared with those of the free one. Tannase was produced intracellularly by the xerophilic fungus Aspergillus niger GH1 in a submerged fermentation system. Enzyme was recovered by cell disruption and the crude extract was partially purified. T...

A biochemical characterization of the tannase from a Paecilomyces variotii strain isolated in São Paulo, Brazil was carried out. Paecilomyces variotii is a strain obtained from the screening of five hundred fungi that were tested for their production of tannase. The enzyme produced was partially purified using ammonium sulfate precipitation followed by ion exchange chromatography, diethylaminoe...

An extracellular tannase was produced from solid-state cultures of Aspergillus niger. The enzyme was purified to homogeneity from the cell-free culture broth by preparative isoelectric focusing and by FPLC using anion-exchange and gel-filtration chromatography. SDS-PAGE analysis as well as gel localization studies of purified tannase indicated the presence of two enzyme forms, with molecular ma...

Extracellular tannase production by Trichophyton rubrum has been evaluated using submerged fermentation (SmF) at different abiotic factors like pH, temperatures and biotic factors like carbon, nitrogen and concentration of gallic acid. T. rubrum produced the highest tannase level at a temperature of 30°C where the enzyme production was 23.52 U/mL and pH of 5.5 where enzyme production was 32.62 ...

Modified solid-state fermentation (MSSF) of tannin-rich substrates for production of tannase and gallic acid was carried out using fungal co-cultures. Tannin acyl hydrolase or tannase is an enzyme produced extracellularly in solid state fermentation by various fungi. The solid-state fermentation offers a number of advantages over submerged and liquid conventional fermentation for the enzyme pro...