objective: the objective of this study is to develop and assess targeted pamam-peg nanocarrier with anti-tag72 nanobody for t-bid gene coding construct delivery into the human colonic adenocarcinoma cells. materials and methods: nanobody (nb) coding sequence was subcloned into psj expression vector for large-scale production and then nb was purified by ni++ affinity chromatography. sds-page an...

background : lung cancer is still the leading cause of cancer- related deaths and most of themâ  are normally diagnosed in late stages, so there is an urgent need to establish reliable tools for the identification at early stages of the disease. in this study, we use an eleven gene signature test to examine lung biopsy specimens for diagnostic, prognostic & therapeutic purposes. methods : bronc...

background human telomerase reverse transcriptase (htert) has become an ideal target for development of anticancer therapy. small interfering rnas (sirnas) are very powerful reagents for gene silencing and show promise for cancer gene therapy. however, only a small number of sirnas have been demonstrated to be effective. for gene therapy targeting htert, it is essential to develop a robust syst...

neuroendocrine tumors (nets) constitute a heterogeneous group of neoplasms including carcinoids, pancreatic neuroendocrine tumors, pituitary tumors, medullary thyroid carcinoma and phaeochromocytomas. the symptoms and the outcome of nets differ considerably between patients depending on several factors. by labelling tracers with a radioisotope, the tracer acts as a carrier to deliver the radioa...