Embryonic development can be partially recapitulated in vitro by differentiating human embryonic stem cells (hESCs). Thalidomide is a developmental toxicant in vivo and acts in a species-dependent manner. Besides its therapeutic value, thalidomide also serves as a prototypical model to study teratogenecity. Although many in vivo and in vitro platforms have demonstrated its toxicity, only a few ...

Abstract: This study aimed to evaluate effectiveness of the chick cardiomyocyte micromass (MM) system for prediction of teratogenecity of common environmental factors and prevention of such effects by addition of multivitamins. White Leghorn 5-day-old embryo hearts were dissected and disassociate to produce a cardiomyocyte cell suspension in Dulbecco's Modified Eagle's Medium. The cultures were...

Accepted: 29/12/2014 Abstract Polycyclic Aromatic Hydrocarbons (PAHs) are ubiquitous environmental contaminants and found almost everywhere. Most of PAHs are formed during the incomplete combustion of organic matter such as wood, petroleum product, coal and tar. These chemicals are biologically active and pose a risk for human health because of their potentially toxicity such as carcinogenicity...

background: vanadium is an important environmental and industrial pollutant. it has a status of reproductive toxicant and is reported to cross placental barrier. objective: the current study was performed to assess the therapeutic efficacy of tiron and its combination with selenium against vanadium induced toxicity in lactating and suckling rats. materials and methods: rats were exposed to vana...

An organophosphorus insecticide, chlorpyrifos was tested for the embryotoxicity and teratogenecity in mice. Different sublethal doses of the insecticide i.e. 20, 40, 60 and 80ug/gm body weight were prepared by dissolving it in corn oil in such a way that each 0.1ml of the solution contains desired concentration. The doses were given orally on day 6 of gestation to different experimental groups....

Rats were orally fed with different doses viz. 9.6, 12 and 15 mg/kg/d from GD 0-20 and examined for evidence of fetotoxicity and teratogenecity to evaluate the potential effects of technical chlorpyrifos (97%). Fetotoxic effects were not observed at tested dose levels as evidenced by number of implantations, number of corpora lutea and live fetuses/dam, but significant alterations were noted in...

The folic acid antagonists, methotrexate and aminopterin, are known to be teratogenic in humans. The critical period for their teratogenecity is suspected to be between 6 to 8 weeks post-conception. Fetal exposure from 10 to 32 weeks weeks post-conception to methotrexate alone or in combination with other anti-cancer drugs has not resulted in obvious teratogenic effects. Methotrexate is often u...

Valproic acid (VPA) is used worldwide to treat epilepsy, migraine headaches, and bipolar disorder. However, VPA is teratogenic and in utero exposure can lead to congenital malformations. Using inbred C57BL/6J (B6) and DBA/2J (D2) mice, we asked whether genetic variation could play a role in susceptibility to VPA teratogenesis. Whereas B6 fetuses were more susceptible than D2 fetuses to digit an...