A molecular study was undertaken to detect Theileria ovis, Theileria lestoquardi and Theileria annulata in sheep and tick vectors. Investigation was conducted from 2010 to 2011 in the south of Khorasan Razavi Province, Iran. A total of 150 blood samples were collected from 30 different sheep flocks. In addition, ixodid ticks were sampled from the same flocks. The stained blood smears were micro...

BACKGROUND The present study was carried out to investigate the accurate status of ovine Theileria infection in sheep from Ahvaz and surrounding region, a tropical area southwest Iran. METHODS A PCR-RFLP method based on 18S ribosomal RNA gene was designed which could detect and differentiate Theileria and Babesia spp. and also differentiate main Theileria species in sheep at the same time. 11...

This study was aimed to determine the infection rate and vectors of Theileria lestoquardi in goats from West Azerbaijan province, Iran. A total of 400 blood samples were collected from 40 randomly selected flocks in the study area from June to September, 2014. Out of 400 blood samples examined using microscopic examination, a number of 14 goats (3.50%) were positive for Theileria spp., whereas ...

A total of 200 whole blood samples along with thin blood samples smears and 100 samples of ticks from 20 flocks of sheep from different localities of district Lahore with a history of tick infestation , relapse of fever and anemia were screened for the prevalence of Theileria species during spring and summer seasons in 2007. On microscopic examination 44/200 (22%) samples were positive for Thei...

the aim of this study was to detect and differentiate theileria annulata and t. lestoquardi (hirci) by pcr. members of the genus theileria are tick-borne hemoprotozoan parasites those cause fatal and enervating diseases of cattle and sheep in iran . in order to develop a specific method for detecting and identification of theileria species, specific primers from the surface protein (sp) sequenc...

  The aim of this study was to detect and differentiate Theileria annulata and T. lestoquardi (hirci) by PCR. Members of the genus Theileria are tick-borne hemoprotozoan parasites those cause fatal and enervating diseases of cattle and sheep in Iran . In order to develop a specific method for detecting and identification of Theileria species, specific primers from the surface protein (SP) seque...

The establishment of good experimental models for Theileria sp. infection is important for theileriosis research. Routinely, infection of ticks is accomplished by feeding on parasite-infected animals (sheep, cows and horses), which raises practical and ethical problems, driving the search for alternative methods of tick infection. Artificial tick feeding systems are based mainly on rearing tick...

The prevalence of Theileria lestoquardi antibodies in Sudanese sheep from nine geographical areas in Sudan was determined using indirect fluorescent antibody "IFA" test. Out of 315 samples examined, 51 (16.2%) were found positive and ranged between 23.4% in River Nile State and 10% in Kasala and Darfour Provinces with an overall prevalence of 16.2% indicating widespread distribution of the infe...

Identification of attenuation markers of a Theileria lestoquardi cell line to be used for the development of live vaccine against malignant ovine theileriosis Inaugural-Dissertation zur Erlangung der tiermedizinischen Doktorwürde

Ticks and tick-borne diseases, including theileriosis, constitute a major constraint to livestock production. Two species, known as Theileria lestoquardi and Theileria ovis, are suspected to contribute to ovine theileriosis in Iran. However, the epidemiological aspects of ovine theileriosis are poorly understood in this country. In a survey, designed to identify Theileria species in sheep, 52 (...