background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...

BACKGROUND Cell-free DNA (CFDNA) in the plasma/serum of patients with cancer demonstrates tumour-associated genetic alterations, offering possibilities for diagnosis, prognostication and disease monitoring. There is wide variation in the reported levels of CFDNA, associated with different methods used to collect, process and analyze blood samples. We therefore evaluated different aspects of lab...

BACKGROUND Discovery of short cell free fetal DNA (cffDNA) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. The use of cffDNA to set up noninvasive prenatal test is limited due to the low concentration of fetal DNA in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. The aim of this study was to eva...

INTRODUCTION The human monocytic leukemia cell line THP-1 is a widely used model for investigating monocyte and macrophage biology. Successful transfection of THP-1 monocytes with subsequent phorbol 12-myristate 13-acetate (PMA)-induced differentiation into macrophages is not a trivial matter, because according to previous transfection protocols, cell viability is lost almost completely within ...

Macrophages are an important part of the cellular immune system and play a key role during immune responses. Thus, macrophages are interesting targets in basic and clinical research. Primary monocytes or monocyte-derived macrophages do not proliferate on a suitable scale so that their use for functional studies in vitro is limited. Immortal proliferating cell lines, such as the human THP-1 mono...

in multi-step organic syntheses, the protection and deprotection of tetrahydropyranyl (thp) ethers is one of the most frequently used methods. over the years, different methods have been used from various catalytic systems for the protection of hydroxyl groups as thp ethers and their deprotection. herein we have reported that various alcohols and phenols have been efficiently converted to the c...

In multi-step organic syntheses, the protection and deprotection of tetrahydropyranyl (THP) ethers is one of the most frequently used methods. Over the years, different methods have been used from various catalytic systems for the protection of hydroxyl groups as THP ethers and their deprotection. Herein we have reported that various alcohols and phenols have been efficiently converted to the c...

The meroterpenoids fornicin A, D, and ganodercin found in mushrooms of the Ganoderma genus, have been prepared a concise divergent synthesis route. characteristic unsaturated γ-ketoacid moiety was obtained via an optimized step-wise aldol condensation between two readily accessible building blocks. THP-protection phenolic hydroxyl group under basic conditions developed, protocol that adds to ve...

Introduction: Introduction: Molecular components within the microenvironment act upon cell growth, survival/apoptosis, and proliferation. Immune system cells respond to molecules produced by the tumor and released in the surrounding microenvironment, such as cytokines, chemokines, and growth factors. This study aimed to identify the effects of tumor environment on monocyte-macrophage cell linea...

Introduction: Introduction: Molecular components within the microenvironment act upon cell growth, survival/apoptosis, and proliferation. Immune system cells respond to molecules produced by the tumor and released in the surrounding microenvironment, such as cytokines, chemokines, and growth factors. This study aimed to identify the effects of tumor environment on monocyte-macrophage cell linea...