background lung cancer is one of the most common causes of death worldwide. although smoking and environmental pollutants are the most important risk factors of lung cancer, the role of infectious causes should also be considered in the pathogenesis and progress of lung cancer. objectives this study examined the relationship between helicobacter pylori and lung cancer through serology, real-tim...

vacuolar h+-translocation pyrophosphatase (v-ppase) and na+/h+ antiporter (v-na+/h+) are important transporters in plant cells and have essential roles against abiotic stresses. in this study, the effects of salt stress were surveyed on the transcription of v-ppase pump and na+/h+ antiporter genes at early times of nacl treatment in roots of halophytic grass leptochloa fusca through the real ti...

Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...

We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...

Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...

The image on this cover is of an OpenArray ® plate which is primarily used for mid-density real-time PCR on the QuantStudio™ 12K Flex system. The figure above shows the commonly used formats for real-time PCR.