There is a dizzying array of fluorescent probes now commercially available to monitor cellular processes, and advances in molecular biology have highlighted the ease with which proteins can now be labelled with fluorophores without loss of functionality. This has led to an explosion in the popularity of fluorescence microscopy techniques. One such specialized technique, total internal reflectio...

Structural characterization of protein-protein complexes is required to fully understand biological processes. However, such studies can be difficult, particularly when the interactions are transient. In some cases, the covalent linking of weakly interacting binding partners has been shown to facilitate structural studies. Here, we used this approach to investigate, by X-ray crystallography, th...

We investigate the dependence of the total-infrared (TIR) to UV luminosity ratio method for calculating the UV dust attenuation A(UV ) from the age of the underlying stellar populations by using a library of spectral energy distributions for galaxies with different star formation histories. Our analysis confirms that the TIR/UV vs. A(UV ) relation varies significantly with the age of the underl...

In plants, light signals caused by the presence of neighbors accelerate stem growth and flowering and induce a more erect position of the leaves, a developmental strategy known as shade-avoidance syndrome. In addition, mutations in the photoreceptors that mediate shade-avoidance responses enhance disease susceptibility in Arabidopsis thaliana. Here, we describe the Arabidopsis constitutive shad...

Introduction: Chemical weathering and petrologic trends of the martian surface have been estimated from thermal infrared (TIR) spectral models from the Thermal Emission Spectrometer (TES) and Mini-TES on the Spirit and Opportunity rovers [1,2]. Interpretations of chemical data from APXS on the MER rovers and TIR data from TES and the Thermal Emission Imaging System (THEMIS) suggest planet-wide ...

We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.

Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R) play crucial roles in host innate immune response against microbial infections. These receptors share a conserved cytoplasmic domain, the Toll/interleukin-1 receptor (TIR) domain, which is required for signaling through these receptors. Structural information on the TIR domains will be essential for understanding the molecular basis ...

The HYPXIM concept introduces the next hyperspectral space sensors generation with : enhanced spatial resolution from 15 m to 8 m, higher revisit frequency available for Security and Defence actors, miniaturization allowing a microsatellite to achieve a high-resolution hyperspectral low cost demonstration mission by 2018, a new TIR Hyperspectral capability, multi-sensors fusion products using o...