To establish an intimate interaction with the host epithelial cell surface, enteropathogenic Escherichia coli (EPEC) produces Tir, a bacterial protein that upon translocation and insertion into the epithelial cell membrane constitutes the receptor for intimin. The tir gene is encoded by the locus for enterocyte effacement (LEE), where it is flanked upstream by orf19 and downstream by the cesT a...

Introduction: Hyperglycemia, hypoglycemia and increased glucose variability are independently associated with increased risk of death in critically ill adults. The relationship between time in targeted blood glucose range (TIR) and mortality is not well described and may be a factor that has confounded the results of the major interventional trials of intensive insulin therapy. Methods: We cond...

Our goal was to determine effects of low-level-laser-therapy (LLLT) on skin water and tissue indentation resistance (TIR) in patients with arm (N = 38) or leg (N = 38) lymphedema. Skin water was determined from tissue dielectric constant (TDC) measurements and TIR determined from measurements of force resulting from tissue indentations of 3-4 mm. A limb-location with fibrosis was identified by ...

A two-step approach to enhance the resolution of remote sensing thermal infrared (TIR) images is proposed in this paper. For difference in imaging principles between TIR image and optical images, traditional image fusion techniques, such as component substation and MRA methods will not be proper. In our study, we use Extreme Learning Machine (ELM) to regress the relationship between TIR image a...

Expression of the bacteriophage Mu mom gene is subject to posttranscriptional regulation by the phage com gene product. We have used mom-lacZ translational fusion genes to define the sequence requirements for stimulation of mom expression by Com. We show that the mom translation initiation region (TIR) is inactive in the absence of Com. We suggest that this repressed state is due to mRNA second...

Land surface temperature (LST) retrieved from satellite thermal sensors often consists of mixed temperature components. Retrieving subpixel LST is therefore needed in various environmental and ecological studies. In this paper, we developed two methods for downscaling coarse resolution thermal infrared (TIR) radiance for the purpose of subpixel temperature retrieval. The first method was develo...

Most thermal infrared (TIR) tracking methods are discriminative, which treat the tracking problem as a classification task. However, the objective of the classifier (label prediction) is not coupled to the objective of the tracker (location estimation). The classification task focuses on the between-class difference of the arbitrary objects, while the tracking task mainly deals with the within-...

The importance of the Escherichia coli O157:H7 translocated intimin receptor (Tir) protein in intestinal colonization and the effect of infection with Tir(+) strains on protection against subsequent challenge was studied in adult beef cattle. Cattle were orally inoculated (C1) with a Shiga toxin-2(+)E. coli O157:H7 strain that was Tir(+) or Tir(-), and 42 days later were re-challenged (C2) with...

Attaching and effacing (A/E) lesions and actin polymerization, the hallmark of enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC) and Citrobacter rodentium (CR) infections, are dependent on the effector Tir. Phosphorylation of Tir(EPEC/CR) Y474/1 leads to recruitment of Nck and neural Wiskott-Aldrich syndrome protein (N-WASP) and strong actin polymerization in cultured c...

In the current study, we show that macrophages adaptively resist anthrax lethal toxin (LT) through a toxin-activated process termed toxin-induced resistance (TIR). TIR was triggered by pretreatment of RAW 264.7 or J774A.1 macrophages with a low dose of LT for at least 6 h, which resulted in resistance to high doses of LT for 96 h. Activation of TIR required functional toxin, because LT subunits...