Background and Aim: Mycobacterium tuberculosis is a health problem in countries. Despite the global prevalence of tuberculosis and the lack of appropriate drugs, further progress is still needed with the help of modern methods of preparing epitope-based vaccines for tuberculosis. Materials and Methods: In this study, specific T and B cell epitopes required for producing chimeric vaccines with ...

background the 30 kda major secretory protein of mycobacterium tuberculosis (antigen 85b) is a primary vaccine candidate. this secreted antigen induces a protective immune response and stimulates the production of ifn-γ in animal models. objectives the aim of this study was cloning and expression of ag 85b of m. tuberculosis in escherichia coli. materials and methods to produce recombinant ag85...

this study was carried out to control johne’s disease (jd) without interfering with tuberculosis (tb)control programme. a dairy herd (n>1500 heads) was used over a period of 6 years, from 1994 to 1999 forthis investigation. the herd had a history of heavy infection with mycobacterium avium paratuberculosis(map) with culling rates of 1.5 and 5%, in 1991 and 1995, respectively. the mean annual lo...

The emergence of drug-resistant Mycobacterium tuberculosis strains and the widespread occurrence of AIDS demand newer and more efficient control of tuberculosis. The protective efficacy of the current Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccine is highly variable. Therefore, development of an effective new vaccine has gained momentum in recent years. Recently, several M. tuberculo...

conclusions in this experimental study, for the first time in iran the expression and purification of this recombinant protein was done successfully. this recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations. results the highest expression of the cytochrome p450 cyp141 protein was obtained by the addition of 1 mm of isopropyl β-d-1-thiogal...

Submit Manuscript | http://medcraveonline.com Abbreviations: BCG: Bacille Calmette-Guerin Vaccine; CI: Confidence Interval; CD4, CD8: A Type of White Blood Cell Help to Defend the Body from Infections; DNA: Deoxyribonucleic Acid; EPI: Expanded Program on Immunizations; GAVI: Global Alliance for Vaccines and Immunization; HIV: Human Immunodeficiency Virus; IAVI: International AIDS Vaccine Initia...

Tuberculosis remains a major public health problem around the world. Because the Mycobacterium bovis Bacilli-Calmette-Guerin (BCG) vaccine fails to protect adults from pulmonary tuberculosis, there is an urgent need for improved vaccine formulations. Unlike BCG, recombinant vaccines purified from bacterial expression vectors, as well as naked DNA, require an additional adjuvant. Recent improvem...

the identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. this study was designed for cloning and expression of esat-6 as a potent antigen of mycobacterium tuberculosis. selected gene (rv3875) was amplified by pcr and product was ligated into expressing plasmid vector pqe30 and recombinant pqe30-es plasmid ...