PURPOSE TUNEL assay is widely used to evaluate cell death. Quantification of TUNEL-positive (TUNEL+) cells in tissue sections is usually performed manually, ideally by two masked observers. This process is time consuming, prone to measurement errors, and not entirely reproducible. In this paper, we describe an automated quantification approach to address these difficulties. METHODS We develop...

BACKGROUND Modes of cell death have been defined morphologically as apoptosis and oncosis. Infarcted myocytes have been reported to show apoptosis, as revealed by DNA fragmentation by DNA ladder and by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) at the light microscopic level. We investigated whether TUNEL-positive infarcted myocytes have apoptotic or o...

BACKGROUND AND PURPOSE Early thrombolytic therapy is encouraged in hyperacute stroke, although this might result in delayed reperfusion injury. Our purpose was to investigate the serial changes in cerebral perfusion following transient ischemia by means of MR imaging and to correlate them with the histologic findings obtained by using the TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay...

The death regulation of damaged pulp cells after cavity preparation is not well-known. In this study, we examined whether apoptosis is associated with the death regulation of damaged pulp cells. In normal rat molars, terminal deoxynucleotidyl transferase-mediated labeling (TUNEL)-positive cells were not observed. Just after surgery, odontoblasts under cavities were TUNEL-positive, and these sig...

Alcohol-sensitive neuronal cell loss, which has been reported in the superior frontal cortex and hippocampus, may underlie the pathogenesis of subsequent cognitive deficits. In the present study, we have used the TUNEL labeling to detect the DNA damage in human alcoholic brains. Seven out of eleven alcoholics exhibited TUNEL-positive cells in both superior frontal cortex and hippocampus, which ...

Materials and methods: A total of 26 C57BL/6J mice were exposed to 75 ± 2% oxygen from postnatal day 7 to 12. On day 12, 12 mice (group C) were injected with 0.1 μg intravitreal octreotide acetate (IVOA) and 14 mice (group D) were injected with 0.05 μg IVOA, in the right eye. The contralateral eyes were injected with 1 μL isotonic saline (control group, group B). Four mice were used as negative...

Abstract Background The routine semen analysis fails to detect sperm DNA damage which contributes the majority of male factor infertility. Sperm fragmentation test (DFI) measures damage. Blastocyst formation is an important step in IVF ± ICSI. At present, literature lacks any data that correlates DFI and blastocyst formation. Main body abstract We searched MEDLINE other databases till 2020 for ...

Programmed cell death is a combined intracellular process. For the activation of apoptosis as proapoptotic genes and antiapoptotic ones are needed as well. There are several methods of apoptosis assessment but in laryngeal cancer prognostic value of apoptotic index is still unclear. 44 patients with laryngeal cancer who received surgical treatment in ENT Department of Medical University of Lodz...