Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile donors comparing the sperm chromatin structure assay (SCSA), TdT-mediated-dUTP nick end labeling (TUNEL), the sperm chromatin dispersion (SCD) test, and acridine orange staining technique (AOT). The TUNEL...

introduction: among the endocrine diseases, hypothyroidism is the commonest after diabetes.thyroid hormones (t3,t4) are essential for genital organs function. apoptosis process in ovarian cells plays a significant role of development in ovarian follicles. the aim of the present study was determine the apoptotic index induced by hypothyroidism in rat ovarian follicles. materials and methods: in ...

Introduction: Among the endocrine diseases, hypothyroidism is the commonest after diabetes.Thyroid hormones (T3,T4) are essential for genital organs function. Apoptosis process in ovarian cells plays a significant role of development in ovarian follicles. The aim of the present study was determine the apoptotic index induced by hypothyroidism in rat ovarian follicles. Materials and Method...

Objective(s): The aim of this study was to determine the protective role of ascorbic acid on apoptosis and proliferation of spermatogonia and primary spermatocyte cells after malathion administration as an organophosphate pesticide in rat testis.Materials and Methods: Thirty male Wistar rats were randomly divided into five groups of 6 ra...

the aim of this study was to evaluate and compare the sensitivity of plant bioassays:tradescantia-micronucleus (trad-mcn) tests using bnl 02 and 4430 clones, and crepis capillaris hairyroots chromosome aberrations test. the evaluation of the sensitivity of the tests was based on the example of the analysis of the genotoxicity of water samples from two natural water reservoirs in poland: the raw...

To investigate the in vitro inhibitory effect of rAd-ODC/Ex3as on human esophageal carcinoma cells. The infection rate of rAd-ODC/Ex3as was measured with the aid of GFP expression. Western Blot technique was used to observe the inhibition of ODC expression in infected tumor cells. The malignant phenotype of Eca109 cell line was assessed by growth curve. TUNEL was used to analyze cell apoptosis....

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times o...

Background: In this study we evaluate the effects of different doses of GDF9 with fixed dose of BMP15 on quality of in vitro maturated sheep oocytes. Materials and Methods: Good ovine Cumulus-oocyte complexes (COCs) were first transferred to Pre-IVM medium, and then to three IVM mediums supplemented with 10%v/v BMP15 and: 1) 175 ng/ml 2) 131 ng/ml or 3) 219 ng/ml GDF9. After maturation period, ...