With recent advancements of both biological and computer technologies, large-scale experiments are on the point of being undertaken. However, it is unlikely that the experiments will be sufficient to replace traditional studies where a single molecule is examined. For instance, several methods attempt to reconstruct a gene regulatory network from expression measurement data do not explore the p...

Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We sh...

Protein-protein interactions (PPIs) play crucial roles in protein function for a variety of biological processes. Data from large-scale PPI screening has contributed to understanding the function of a large number of predicted genes from fully sequenced genomes. Here, we report the systematic identification of protein interactions for the unicellular cyanobacterium Synechocystis sp. strain PCC6...

Mutations A49P and Delta47-53 at the T loop of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J. Bacteriol. 179: 4342-4353, 1997). We show here that these mutations also impair interactions between PII and NtrB in ...

1 Abstract: In the frame of the FAST20XX project under the supervision of ESA, two hybrid rocket engines are developed at Université Libre de Bruxelles (ULB) in collaboration with the Royal Military Academy of Belgium to assess the performance of paraffin/N2O propellants. The goal behind the study is to measure the regression rate and optimize the size of ALPHA, which is a sub-orbital vehicle d...

Introduction The two-hybrid system (THS), first introduced by Fields and Song (1989) (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest. Here, we present not a review of the current THS technology but, rather, a comprehe...

As the number of specified bits of different test patterns varies widely in a deterministic test cube, all test patterns are concatenated to a data flow. The original test data can be re-divided by 2 to the power of integer according to the successful characteristics of the LFSR coding, whose length equals to the power of 2 in every division. In the new test approach, the numbers of specific bi...

We present a sharp interface method for computing incompressible immiscible two-phase flows. It couples the Level-Set and Volume-of-Fluid techniques and retains their advantages while overcoming their weaknesses. It is stable and robust even for large density and viscosity ratios on the order of 1000 to 1. The numerical method is an extension of the second-order method presented by Sussman (200...

4-tert-Octylphenol (4-t-OP) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. 4-t-OP completely disappeared after 5 days of treatment and the activities of ligninolytic enzymes, laccase and manganese peroxidase (MnP), were detected during this period, thus suggesting that the disappearance of 4-t-OP ...