A PCR-DGGE primer pair, Tyl2F-Tyl4R, specific to plant parasitic and fungivorous nematodes was designed based on the 18S rRNA gene. The results of community analysis using the primers showed that they are specific to the order Tylenchida. This primer pair detected species belonging to Tylenchida with high sensitivity and high resolution. The number of detected species of plant parasitic and fun...

A survey of the nature and phylogenetic distribution of nematode vulval appendages revealed 3 major classes based on composition, position, and orientation that included membranes, flaps, and epiptygmata. Minor classes included cuticular inflations, protruding vulvar appendages of extruded gonadal tissues, vulval ridges, and peri-vulval pits. Vulval membranes were found in Mermithida, Triplonch...

Pratylenchoides camachoi n. sp. from rosemary (Rosmarinus officinalis L.) in Sierra De Cazorla in southeastern Spain is described and illustrated. Pratylenchoides camachoi n. sp. is a bisexual species characterized by a cylindroid, sometimes clavate-shaped, male tail with a uniquely shaped smooth, rounded terminus. Females are characterized by a short esophageal overlap and six lines in the lat...

Malenchus truncatus n. sp. and M. bryanti n. sp. from Michigan are describes and presented es the first record of Malenchus in North America. M. acarayensis Andrassy, 1968 is recorded and males of this species described. Notes on the geographical distribution of Malenchus and a key to the species are given.

Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences Sergei A. SUBBOTIN 1,2,∗, Dieter STURHAN 3, Vladimir N. CHIZHOV 2, Nicola VOVLAS 4 and James G. BALDWIN 1 1 Department of Nematology, University of California, Riverside, CA 92521, USA 2 Institute of Parasitology of RAS, Leninskii prospect 33, 117071, Moscow, Russia 3 I...

From 2011 to 2012, we collected Scutellonema commune from the rhizosphere soil of coconut (cocos nucifera L.) and Scutellonema magniphasma from the rhizosphere soil of Rosa chinensis in Shenyang, China. S. commune was characterized by rounded scutella, which was varied from two annules anterior to the anus and five annules posterior. The lateral field was not areolated at the level of scutella....

Stability of Heterodera glycines (Tylenchida: Heteroderidae) juvenile hatching from eggs obtained from different sources of soybean, Glycine max Edward P. MASLER 1,∗, Patricia A. DONALD 2 and Sandra SARDANELLI 3 1 Nematology Laboratory, Agricultural Research Service, Beltsville, MD 20705, USA 2 Crop Genetics and Production Research Unit, Agricultural Research Service, Jackson, TN 38301, USA 3 P...

Entomoparasitic nematodes are natural control agents for many insect pests, including fleas that transmit Yersinia pestis, a causative agent of plague, in the natural foci of this extremely dangerous zoonosis. We examined the flea samples from the Volga-Ural natural focus of plague for their infestation with nematodes. Among the six flea species feeding on different rodent hosts (Citellus pygma...