background: ubiquitin, an 8.5 kda peptide that marks other proteins for proteasomal degradation, tags defective sperm dur­ing epididymal passage. thus, sperm ubiquitination is a universal marker for sperm defects and can be used as a sperm func­tion test. the objective of the present study was to examine the relationships between sperm ubiquiti­nation and clinical se­men parameters, using simpl...

Innate immunity is an evolutionary ancient defence strategy that serves to eliminate infectious agents while maintaining host health. It involves a complex network of sensors, signaling proteins and immune effectors detect the danger, then relay execute programme. Post-translational modifications relying on conserved ubiquitin ubiquitin-like are integral part system. Studies using invertebrate ...

Ubiquitination (a.k.a. ubiquitylation) is a protein post-translational modification that typically requires the sequential action of three enzymes: E1 (ubiquitin-activating enzymes), E2 (ubiquitin-conjugating and E3 (ubiquitin ligases) [28]. proteins can target them for proteasomal degradation, or modulate cellular processes including cell cycle progression, transcriptional regulation, DNA repa...

ubiquitin - proteasome system (ups), the major protein degradation pathway in the cells, typically degrades short - lived and damaged proteins and regulates growth and stress responses. this pathway is altered in various cancers, including acute lymphoblastic leukemia (all). all begins with a change in bone marrow cells and is the most common type of leukemia in children under 15 years. ube2q1 ...

Ubiquitin-40S ribosomal protein S27a (RPS27A), ubiquitin-like Fubi, and (S30FAU) are ubiquitin-related proteins that involved in the regulation of immune-related functions such as cell cycle, expression, apoptosis. This study aimed to confirm molecular characteristics, gene expression analysis, antibacterial activity RPS27A S30FAU identified from starry flounder (15 flounders 128.7 ± 18.2 g). A...

The NF-?B transcription factor is involved in inflammation and cell proliferation, survival, transformation. It a heterodimer made of p50 or p52 member the Rel family proteins. are derived from limited ubiquitin- proteasome-mediated proteolytic processing larger precursors p105 p100, respectively. Both can be either processed completely degraded by ubiquitin-proteasome system. Previous work our...

Background: Activation of the ubiquitin-proteasome pathway in various malignancies, including colorectal cancer, is established. This pathway mediates the degradation of damaged proteins and regulates growth and stress response. The novel human gene, UBE2Q2, with a putative ubiquitin-conjugating enzyme activity, is reported to be overexpressed in some malignancies. We sought to investigate the ...

Background Recent studies suggested Contactin-associated protein-like 2 (CNTNAP2) as a novel risk gene for Alzheimer’s disease (AD). Reduced expression levels of CNTNAP2 were found in the brains AD patients. However, mechanisms underlying protein degradation remain elusive. Method HEK and N2a cells transfected with human plasmid treated cycloheximide, lysosome inhibitors, proteasome inhibitors....

Hypoxia is an important stress for organisms, including plants and mammals. In plants, hypoxia can be the consequence of flooding causes crop losses worldwide. mammals, may result pathological conditions. Understanding regulation responses to offers insights into novel approaches improvement, particularly development flooding-tolerant crops producing better therapeutics hypoxia-related diseases...

Small ubiquitin-like modifiers (SUMO) and ubiquitin are frequent post-translational modifications of proteins that play pivotal roles in all cellular processes. We previously reported mass spectrometry-based proteomics methods enable profiling lysines modified by endogenous SUMO or an unbiased manner, without the need for genetic engineering. Here we investigated applicability precursor filteri...