Abstract von Willebrand factor (VWF) is a large adhesive multimeric protein involved in hemostasis. The larger the size (or number of VWF multimers), greater functionality protein. A deficiency or defect can lead to disease (VWD) and cause bleeding. Conversely, an increase may create environment that promotes thrombosis. ADAMS-13 (a disintegrin metalloproteinase with thrombospondin type 1 motif...

Background Von Willebrand factor (VWF) contains a number of free thiols, the majority which are located in its C-domains, and these have been shown to alter VWF function, However, impact thiols on function following acute exposure collagen under high pathological shear stress has not determined. Methods were blocked with N-ethylmaleimide flow assays performed rates determine platelet capture bi...

Abstract The primary polypeptide sequence of von Willebrand factor (VWF) includes an N-terminal 741-amino acid VWF propeptide (VWFpp). In cells expressing VWF, the VWFpp performs two critical functions. Golgi, mediates intermolecular disulfide linkages that generate high-molecular-weight multimers. Subsequently, VWFpp, which is proteolytically cleaved from mature by furin, functions to endothel...

Abstract Recent studies have reported that patients with von Willebrand disease treated perioperatively a factor (VWF)/factor VIII (FVIII) concentrate ratio of 2.4:1 (Humate P/Haemate P) often present VWF and/or FVIII levels outside prespecified target necessary to prevent bleeding. Pharmacokinetic (PK)-guided dosing may resolve this problem. As clinical guidelines increasingly recommend aiming...

OBJECTIVE von Willebrand factor (VWF), which is synthesized in endothelial cells and megakaryocytes, is known to worsen stroke outcome. In vitro studies suggest that platelet-derived VWF (Plt-VWF) is biochemically different from the endothelial cell-derived VWF (EC-VWF). However, little is known about relative contribution of different pools of VWF in stroke. APPROACH AND RESULTS Using bone m...

BACKGROUND The coagulation protein von Willebrand Factor (VWF) is known to be elevated in pregnancy. However, the timing and nature of changes in VWF and associated parameters throughout pregnancy are not well understood. OBJECTIVES To better understand the changes in VWF provoked by pregnancy, we studied VWF-associated parameters in samples collected over the course of healthy pregnancies. ...

Abstract Background Von Willebrand disease (VWD) is an autosomal recessive congenital bleeding disorder with deficiency or dysfunction of von Willebrand factor (VWF). The gene encoding for the VWF is located on chromosome 12, which is 178 Kb with 52 exons. Various mutations of this gene is responsible for the clinical features of VWD, but some single nucleotide polymorphisms make the molecu...