The Wnt-Wingless (Wg) pathway regulates development through precisely controlled signaling. In this study, we show that intracellular trafficking regulates Wg signaling levels. In Drosophila melanogaster cells stimulated with Wg media, dynamin or Rab5 knockdown causes reduced Super8XTOPflash activity, suggesting that internalization and endosomal transport facilitate Wg signaling. In the wing, ...

Wnt signaling plays many important roles in animal development. This evolutionarily conserved signaling pathway is highly regulated at all levels. To identify regulators of the Wnt/Wingless (Wg) pathway, we performed a genetic screen in Drosophila. We identified the microRNA miR-8 as an inhibitor of Wg signaling. Expression of miR-8 potently antagonizes Wg signaling in vivo, in part by directly...

Abstract The impacts of future climate change on the watershed streamflow and total dissolved nitrogen (TDN) fluxes upstream Danjiang River were estimated. newest shared socioeconomic pathways (SSP) in CMIP6 used as a scenario. ensembles downscaled GCM outputs from WorldClim information. A combined modeling approach is proposed, including Long Ashton Research Station Weather Generator (LARS-WG)...

background: municipal solid waste (msw) is the natural result of human activities. msw generation modeling is of prime im­portance in designing and programming municipal solid waste management system. this study tests the short-term pre­diction of waste generation by artificial neural network (ann) and principal component-regression analysis. methods: two forecasting techniques are presented in...

climate change is a phenomenon that has made the most concern in communities and decision making centers. this phenomenon has important effects on different aspects of human life. one of the most important crops that are affected by climate change is wheat. accordingly, data and information of northern khorasan have been used to study the effects of climate change on rain-fed wheat yield. for t...

BACKGROUND Secreted signaling proteins of the Wingless (Wg)/Wnt, Hedgehog and bone morphogenetic protein (BMP)/Decapentaplegic (Dpp) families function as morphogens to control growth and pattern formation during development. Although these proteins have been shown to act directly on distant cells in the developing limbs of the fruit fly Drosophila, little is known about how ligand gradients for...

Intestinal stem cell (ISC) self-renewal and proliferation are directed by Wnt/β-catenin signaling in mammals, whereas aberrant Wnt pathway activation in ISCs triggers the development of human colorectal carcinoma. Herein, we have utilized the Drosophila midgut, a powerful model for ISC regulation, to elucidate the mechanisms by which Wingless (Wg)/Wnt regulates intestinal homeostasis and develo...