PURPOSE To investigate the expression of laminin-5 (LM5) and its receptors by human corneal endothelial cells (HCECs) and whether recombinant human LM5 influences adhesion, proliferation, and migration of cultured HCECs. METHODS The expression of LM5 and its receptors was examined in human donor corneas by immunohistochemistry, reverse transcription-polymerase chain reaction, and flow cytomet...

Schnackenberg, Bradley J., Stacie M. Jones, Crystal Pate, Brian Shank, Laura Sessions, Laura M. Pittman, Lawrence E. Cornett, and Richard C. Kurten. The -agonist isoproterenol attenuates EGF-stimulated wound closure in human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol 290: L485–L491, 2006. First published October 14, 2005; doi:10.1152/ajplung.00233.2005.— Asthma is a disease cha...

Introduction: Impaired wound healing in diabetic patients is a major clinical problem, which is associated with significant morbidity and mortality. Estrogen has positive effects on neoangiogenesis, reepithelialization and cell proliferation. In this research, effect of estrogen on wound healing in diabetic male rats was investigated. Methods: This study was performed on male Wistar rats (bo...

BACKGROUND Adipose-derived stem cells (ASCs) are mesenchymal stem cells that have recently been applied to tissue repair and regeneration. Keratinocytes and dermal fibroblasts play key roles in cutaneous wound healing. OBJECTIVE We investigated the paracrine effects of ASCs on HaCaT cells (i.e., immortalized human keratinocytes) and human dermal fibroblasts to explore the mechanism of the eff...

Aloe vera has long been used as a traditional medicine for inducing wound healing. Gingival fibroblasts (GFs) play an important role in oral wound healing. In this study, we investigated the effects of acemannan, a polysaccharide extracted from Aloe vera gel, on GF proliferation; keratinocyte growth factor-1 (KGF-1), vascular endothelial growth factor (VEGF), and type I collagen production; and...

BACKGROUND Trefoil factor 1 (TFF1) mediates mucosal repair and belongs to a highly conserved trefoil factor family proteins which are secreted by epithelial cells in the stomach or colon mucous membrane. TFF1 forms a homodimer via a disulphide linkage that affects wound healing activity. Previous recombinant expressions of TFF1 were too low yield for industrial application. This study aims to i...

PURPOSE Epidermal growth factor (EGF) stimulates migration in corneal epithelial wound healing. The purpose of this study was to investigate the effect of EGF-induced alpha-tubulin deacetylation through activating HDAC6 on migration in corneal epithelial wound healing. METHODS Human corneal epithelial (HCE) cells were cultured in DMEM/F12 medium containing 10% FBS in a 37 degrees C incubator ...

objectives effect of using autologous platelet-rich plasma (prp) on pain reduction in patients with pilonidal abscess, subjected to extensive surgical removal tissue, was investigated through a clinical trial. patients and methods the trial was conducted on two groups from the center for surgical research at mashhad university of medical sciences located at ghaem hospital in mashhad city in 201...

nitric oxide (no) is an important molecule synthesized during wound repair. studies have reported the use of no donors on cutaneous wound repair, but their effects in different phases of healing are not elucidated. the aim of this work was to investigate the effects of topical sildenafil on wounds in rats. sildenafil (25 mg) was topically applied once daily on wound in treatment groups. on days...