نتایج جستجو برای: آنزیم g9a

تعداد نتایج: 12176  

سلول‌های بنیادی مزانشیمی (MSCs)، سلول‌های بنیادی چندتوانی هستند که پتانسیل تمایز به دودمان‌های بافت همبند را داشته و ویژگی‌های منحصربه‌فردی از قبیل تعدیل سیستم ایمنی و ترشح فاکتورهای رشد را دارند. آنزیم هیستون متیل ترانسفراز G9a یکی از فاکتورهای دخیل در کنترل رفتار و ویژگی بسیاری از سلول‌های بنیادی می‌باشد. از این‌رو، بررسی نقش G9a در کنترل رفتار و پتانسیل MSCs دارای اهمیت می‌باشد. MSCs از مغز ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2012
Danielle Bittencourt Dai-Ying Wu Kwang Won Jeong Daniel S Gerke Laurie Herviou Irina Ianculescu Rajas Chodankar Kimberly D Siegmund Michael R Stallcup

Histone H3 lysine-9 methyltransferase G9a/EHMT2/KMT1C is a key corepressor of gene expression. However, activation of a limited number of genes by G9a (independent of its catalytic activity) has also been observed, although the precise molecular mechanisms are unknown. By using RNAi in combination with gene expression microarray analysis, we found that G9a functions as a positive and a negative...

2015
Jie Zhang Pengxing He Yong Xi Meiyu Geng Yi Chen Jian Ding

G9a, a histone methyltransferase, is aberrantly expressed in some human tumor types. By comparing 182 paired colorectal cancer and peritumoral tissues, we found that G9a was highly expressed in colorectal cancer (CRC). Overexpression of G9a promoted CRC cells proliferation and colony formation, whereas knockdown of G9a inhibited CRC cells proliferation. Depletion of G9a increased the rate of ch...

2017
Ruey-Jien Chen Chia-Tung Shun Men-Luh Yen Chia-Hung Chou Ming-Chieh Lin

Research suggests that the epigenetic regulator G9a, a H3K9 histone methyltransferase, is involved in cancer invasion and metastasis. Here we show that G9a is linked to cancer angiogenesis and poor patient survival. Invasive cervical cancer has a higher G9a expression than cancer precursors or normal epithelium. Pharmacological inhibition and genetic silencing of G9a suppresses H3K9 methylation...

2017
Masayuki Yokoyama Tetsuhiro Chiba Yoh Zen Motohiko Oshima Yuko Kusakabe Yoshiko Noguchi Kaori Yuki Shuhei Koide Shiro Tara Atsunori Saraya Kazumasa Aoyama Naoya Mimura Satoru Miyagi Masanori Inoue Toru Wakamatsu Tomoko Saito Sadahisa Ogasawara Eiichiro Suzuki Yoshihiko Ooka Akinobu Tawada Masayuki Otsuka Masaru Miyazaki Osamu Yokosuka Atsushi Iwama

Histone H3 lysine 9 dimethylation (H3K9me2) is mainly regulated by the histone lysine methyltransferase G9a and is associated with the repression of transcription. However, both the role of G9a and the significance of H3K9me2 in hepatocellular carcinoma (HCC) cells remain unclear. In this study, we conducted loss-of-function assay of G9a using short-hairpin RNA and pharmacological interference....

Journal: :The EMBO journal 2013
Lifeng Wang Shiliyang Xu Ji-Eun Lee Anne Baldridge Sean Grullon Weiqun Peng Kai Ge

PPARγ promotes adipogenesis while Wnt proteins inhibit adipogenesis. However, the mechanisms that control expression of these positive and negative master regulators of adipogenesis remain incompletely understood. By genome-wide histone methylation profiling in preadipocytes, we find that among gene loci encoding adipogenesis regulators, histone methyltransferase (HMT) G9a-mediated repressive e...

2014
Xiao-Xue Ke Dunke Zhang Shunqin Zhu Qingyou Xia Zhonghuai Xiang Hongjuan Cui

Histone methylation plays an important role in gene transcription and chromatin organization and is linked to the silencing of a number of critical tumor suppressor genes in tumorigenesis. G9a is a histone methyltransferase (HMTase) for histone H3 lysine 9. In this study, we investigated the role of G9a in neuroblastoma tumor growth together with the G9a inhibitor BIX01294. The exposure of neur...

Journal: :The EMBO journal 2008
Makoto Tachibana Yasuko Matsumura Mikiko Fukuda Hiroshi Kimura Yoichi Shinkai

Methylation of DNA and lysine 9 of histone H3 (H3K9) are well-conserved epigenetic marks for transcriptional silencing. Although H3K9 methylation directs DNA methylation in filamentous fungi and plants, this pathway has not been corroborated in mammals. G9a and GLP/Eu-HMTase1 are two-related mammalian lysine methyltransferases and a G9a/GLP heteromeric complex regulates H3K9 methylation of euch...

2015
Feng Li Jin Zeng Yang Gao Zhenfeng Guan Zhenkun Ma Qi Shi Chong Du Jing Jia Shan Xu Xinyang Wang Luke Chang Dalin He Peng Guo Wei-Guo Zhu

G9a has been reported to highly express in bladder transitional cell carcinoma (TCC) and G9a inhibition significantly attenuates cell proliferation, but the underlying mechanism is not fully understood. The present study aimed at examining the potential role of autophagy in the anti-proliferation effect of G9a inhibition on TCC T24 and UMUC-3 cell lines in vitro. We found that both pharmaceutic...

2015
Chunjing Bian Qiang Chen Xiaochun Yu

The G9a/GLP complex mediates mono- and dimethylation of Lys9 of histone H3 at specific gene loci, which is associated with transcriptional repression. However, the molecular mechanism by which the G9a/GLP complex is targeted to the specific gene loci for H3K9 methylation is unclear. In this study, with unbiased protein affinity purification, we found ZNF644 and WIZ as two core subunits in the G...

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