نتایج جستجو برای: پروتئین cota

تعداد نتایج: 18103  

ژورنال: :زیست شناسی میکروارگانیسم ها 0
پروین زمانی بروجن بلوار آزادی کوچه پست محمد علی آموزگار خسرو خواجه

مقدمه: لاکازها (بنزن دیول اکسیژن اکسیدو ردوکتاز ec 1. 10. 3. 2 ) یکی از اعضا ی خانواده مالتی کوپر اکسیدازها هستند که اکسیداسیون طیف گسترده ای از ترکیبات فنولی را با استفاده از اکسیژن مولکولی کاتالیز می کنند.   مواد و روش ‏‏ ها : ژن کد‏کننده آنزیم لاکاز از سویه gaz23 به‏وسیله پرایمرهای کلونینگ اختصاصی ژن تکثیرشد. محصول pcr در ناقل بیانی ( pet21a ) کلون به باکتری اشریشیا­کلی سویه bl21(de3) انتقال...

Journal: :Journal of bacteriology 1997
M Sonoda K Kitano A Katoh H Katoh H Ohkawa T Ogawa

cotA of Synechocystis sp. strain PCC6803 is a gene involved in light-induced proton extrusion (A. Katoh, M. Sonoda, H. Katoh, and T. Ogawa, J. Bacteriol. 178:5452-5455, 1996). There are two possible initiation codons in cotA, and either long (L-) or short (S-) cotA encoding a protein of 440 or 247 amino acids could be postulated. To determine the gene size, we inserted L-cotA and S-cotA into th...

2014
Søren Brander Jørn D. Mikkelsen Kasper P. Kepp

The limitations of fungal laccases at higher pH and salt concentrations have intensified the search for new extremophilic bacterial laccases. We report the cloning, expression, and characterization of the bacterial cotA from Bacillus clausii, a supposed alkalophilic ortholog of cotA from B. subtilis. Both laccases were expressed in E. coli strain BL21(DE3) and characterized fully in parallel fo...

Journal: :Journal of bacteriology 2001
M F Hullo I Moszer A Danchin I Martin-Verstraete

The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA nor other sporulation proteins are involved. We demonstrate that CotA is a laccase. Syringaldazine, a specific substrate of laccases, reacted with wild-type spores but not with DeltacotA spores. Co...

2013
Jianmei Su Peng Bao Tenglong Bai Lin Deng Hui Wu Fan Liu Jin He

Multicopper oxidases (MCOs) are a family of enzymes that use copper ions as cofactors to oxidize various substrates. Previous research has demonstrated that several MCOs such as MnxG, MofA and MoxA can act as putative Mn(II) oxidases. Meanwhile, the endospore coat protein CotA from Bacillus species has been confirmed as a typical MCO. To study the relationship between CotA and the Mn(II) oxidat...

Journal: :Biochemical systematics and ecology 2001
Balderrama Braca Garcia Melgarejo Pizza De Tommasi N

Triterpenes and anthraquinones from Picramnia sellowii Planchon in Hook (Simaroubaceae) Luisa Balderrama, Alessandra Braca*, Emilia Garcia, Marcela Melgarejo, Cosimo Pizza, Nunziatina De Tommasi a Instituto de Investigaciones Quimicas, Universidad Mayor de San Andres, Calle 27, esq. A. Bello, Cota Cota, La Paz, Bolivia Dipartimento di Chimica Bioorganica e Biofarmacia, Universita di Pisa, Via B...

Journal: :The American journal of occupational therapy : official publication of the American Occupational Therapy Association 2000
R P Cottrell

This article provides a historical review of certified occupational therapy assistant (COTA) education, professional roles, career mobility, and professional development. The evolution of COTA education from 2 hospital-based, 12-week training programs to the current 162 accredited 2-year college degree or professional certificate programs is examined. The expansion of the COTA role from well-de...

2016
Yanzhou Zhang Xunhang Li Zhikui Hao Ruchun Xi Yujie Cai Xiangru Liao

For the more efficient detoxification of phenolic compounds, a promising avenue would be to develop a multi-enzyme biocatalyst comprising peroxidase, laccase and other oxidases. However, the development of this multi-enzyme biocatalyst is limited by the vulnerability of fungal laccases and peroxidases to hydrogen peroxide (H2O2)-induced inactivation. Therefore, H2O2-resistant peroxidase and lac...

Journal: :Acta crystallographica. Section D, Biological crystallography 2002
Francisco J Enguita Pedro M Matias Lígia O Martins Diana Plácido Adriano O Henriques Maria Arménia Carrondo

Bacterial endospores are highly resistant structures that allow survival for long periods of time in adverse environments. The spore-forming Gram-positive bacterium Bacillus subtilis synthesizes a coat around the endospore during development composed of several assembled polypeptides. The role of these components of the spore coat remains unclear; however, some of them appear to be enzymes poss...

Journal: :The Journal of biological chemistry 2002
Ligia O Martins Claudio M Soares Manuela M Pereira Miguel Teixeira Teresa Costa George H Jones Adriano O Henriques

The Bacillus subtilis endospore coat protein CotA shows laccase activity. By using comparative modeling techniques, we were able to derive a model for CotA based on the known x-ray structures of zucchini ascorbate oxidase and Cuprinus cereneus laccase. This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried c...

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