بررسی شیوع کلونیزاسیون مخمری ناحیه پره پوس در کودکان ختنه نشده

Abstract Background and purpose: The yeast fungi including Candida and Malassezia genus are normal flora of the skin of human and male genital region. They could cause balanitis and urinary tract infections. The aim of this study was to determine the prevalence of yeast colonization on preputial area of infant prior to circumcision. Materials and methods: A total of 200 (<one year of age) clinically healthy boy was included in this study. Genomic DNA of all positive yeasts growth was extracted and D1/D2 domains of the large subunit (LSU) ribosomal DNA were amplified using polymerase chain reaction. The yeast species were identified using nucleotide Blast at GenBank sequence database. Results: There were 200 children aged between 4 days- 9 months (mean age 58±49 days). In seven (3.5%) cases the yeast colonization was observed. All yeast isolates were identified as Candida albicans with highest sequence identity (99-100%). Conclusion: The low rate of yeast colonization in preputial area of boys less than one year old calls for circumcision in this age since they are less likely to be affected by yeast colonization.