PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA

نویسندگان

  • HOURI REZVAN From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran
  • KAMRAN MOUSAVI HOSSEINI From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran
  • MOHAMMAD ALI JALILI From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran
  • MOJGAN POURMOKHTAR the Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • RASSOUL DINAR VAND the Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
چکیده مقاله:

As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI buffer, pH 8.1, resulting in recovery of 85 % IgM, 84% IgA and 33 % IgG. The comparison of our results with immunoglobulins' percentage in plasma indicates that IgM and IgA -enrichment was obtained by three times.

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عنوان ژورنال

دوره 17  شماره 4

صفحات  315- 318

تاریخ انتشار 2004-02

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