A PCR-based diagnostic assay was developed for early detection and identification of Aphelenchoides fragariae directly in host plant tissues using the species-specific primers AFragFl and AFragRl that amplify a 169-bp fragment in the internal transcribed spacer (ITS1) region of ribosomal DNA. These species-specific primers did not amplify DNA from Aphelenchoides besseyi or Aphelenchoides ritzem...

Few Aphelenchoides spp. are facultative plant-parasites (foliar and bulb nematodes); three of them are well known in agricultural systems, namely Aphelenchoides besseyi, A. fragariae and A. ritzemabosi. Ten other plant-parasitic species, A. arachidis, A. bicaudatus, A. blastophthorus, A. dalianensis, A. ensete, A. nechaleos, A. paranechaleos, A. saprophilus, A. sphaerocephalus and A. subtenuis,...

The rice white tip nematode, Aphelenchoides besseyi, is the primarily important seed-borne pest of rice and was first recorded in Turkey in 1995. These biological agents, such as an entomopathogenic fungus (EPF), entomopathogenic nematodes (EPN), and bacterial supernatants, have been used against other plant parasitic nematodes but as far as not against A. besseyi. Accordingly, the EPF species,...

BACKGROUND The rice white tip nematode Aphelenchoides besseyi, a devastating nematode whose genome has not been sequenced, is distributed widely throughout almost all the rice-growing regions of the world. The aims of the present study were to define the transcriptome of A. besseyi and to identify parasite-related, mortality-related or host resistance-overcoming genes in this nematode. METHOD...

A simple extraction method was developed for obtaining the white tip nematode, Aphelenchoides besseyi Christie, from single seeds of rice, Oryza sativa. The method was as follows: Individual rice seeds were split longitudinally and then transferred into single pipet tips. Tips containing a split seed were then singly placed upright in glass vials with water to extract the nematodes. This method...

Aphelenchoides besseyi is one of the most important parasitic nematodes in rice plants, capable dormancy seeds. This study aims to determine effects soaking seeds water and pesticides (carbosulfan, benomyl, imidacloprid) on mortality A. besseyi, seed viability, symptoms seedlings. specifically used infected with besseyi. Cold treatment (cwt) at 5-10˚C for 24 hours followed by hot (hwt) 52˚C 10 ...

Polianthes tuberosa is a commercially valuable flower crop in the Mekong Delta of Vietnam that is propagated by the harvesting and planting of bulbs. The cultivation of P. tuberosa is complicated by an endemic nematode infection that damages a high proportion of the plants. Based on morphological criteria and ribosomal RNA gene sequencing, we have determined that the infection is caused by an A...

Aphelenchoides besseyi is a seed-borne plant parasitic nematode and is widely distributed in most rice growing areas. Application of resistant cultivars requires the identification of resistance source, which can then be included in breeding programs to improve the local rice cultivars. Fourteen Iranian rice genotypes including improved and local varieties were evaluated for resistance to A. be...

Some organisms can survive extreme desiccation caused by hypertonic osmotic pressure by entering a state of suspended animation known as osmobiosis. The free-living mycophagous nematode Aphelenchoides besseyi can be induced to enter osmobiosis by soaking in osmolytes. It is assumed that sugars (in particular trehalose) are instrumental for survival under environmental stress. In A. besseyi, two...