We developed and evaluated a two-step PCR-based assay with universal primers and genus- or species-specific primers for the detection of the most prevalent bacterial etiologies of otitis media with effusion (OME) in children from Lebanese hospitals. These etiologies included Haemophilus, Streptococcus, and Moraxella (Branhamella) catarrhalis, which were detected in middle-ear effusion (MEE) sam...

background toluene is a cyclic aromatic hydrocarbon which is widely used as an industrial feedstock and as a solvent. it is one of the major parts of pollution in oil-contaminated environments. objectives the main aim of this study was to isolate and characterize a bacterium with high potential application in toluene bioremediation. materials and methods to isolate a toluene-degrading bacterium...

Successive colonization of the thymus by waves of thymocyte progenitors has been described in chicken-quail chimeras and suggested from studies in mice. In swine, we show that the first CD3epsilon-bearing thymocytes appear on day 40 of gestation (DG40). These early thymocytes were CD3epsilonhigh and belonged to the gammadelta T cell lineage. Mature CD3epsilonhigh alphabeta thymocytes were obser...