نتایج جستجو برای: hybridization
تعداد نتایج: 54161 فیلتر نتایج به سال:
In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
پس از شرح مختصری در مورد تولد دانش سیتوژنتیک (1956) و کاربرد آن در پزشکی (1959) به عنوان آزمایش ارزنده ای برای تشخیص بیماریهای ارثی ناشی از ناهنجاریهای کروموزومی، محدودیت دامنه کارآیی آن، محققین را بر آن داشت روش های نواری banding را کشف و به کار گیرند. این روش توانست بسیاری از مشکلات و محدودیت های قبلی را مرتفع نماید ولی باز در مواردی به ویژه در نمونه هایی که امکان کشت بافت مقدور نبود، همچنین...
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...
Many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. In this study for asessing genetic diversity among Bradyrhizobium japonicum isolates, two different probes (sucA and topA) chosen from the chromosomal genome of Bradyrhizobium strain USDA 110 were designed, evaluated by DNAMAN software and im...
world apricot (prunus armeniaca l.) production is increasing steadily due to breeding of new high yielding cultivars in different countries. more recently, breeding programs have been modified according to consumers’ demands and also improvement in resistance to diseases (sharka, monilinia etc.), frost damages, and determination of self-(in) compatibility. in this study, fourteen apricot breed...
hybrids were produced by crossing female caspian kutum rutilus frisii kutum with male grass carp ctenopharyngodon idella. the genome of eight larvae and parents were studied using microsatellite markers for genetic evaluation and verification. after dna extraction from parent fish and progeny, hybrid heritability of two loci was assessed using two pairs of microsatellite primers. hybridizie...
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...
In this paper an idea for hybridization of conventional vehicles has proposed. The case study performed on one of the common vehicles on country roads i.e. Samand. This vehicle has high production volume but low fuel performance therefore hybridization of it could be attractive for its manufacture. This paper aims that the hybridization idea and its structure to need minimum mechanical modifica...
Among vertebrates, most of the fishes are unique for the lack of acrosome in their spermatozoa. The sperm enters the egg through micropyle of the egg. This has facilitated heterologous fertilization in many fishes and led to hybridization and polyploidization. Hybridization is performed both in the nature and domesticated fishes. Today, some of the hybrids have high growth rate than their paren...
in this study, we explained a nanobiosensor for dna sequence detection, featuring sequence specificity, cost efficiency, speed, and ease of use. without the need for labels or indicators, it may be ideal for the detection of biological agents. this review describes recent advances in electrochemical impedance spectroscopy (eis) with an emphasis on using nanoparticles and nanotechnology. a power...
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