اثر انبارداری طبیعی و زوال تسریع شده بر تغییرات کمی و کیفی پروتئین‌های ذخیره‌ای و آنزیم کاتالاز در بذور نخود (Cicer arietinum L.)

    This experiment was laid out in order to study on effect of natural storage and accelerated ageing on quantity and quality changes in storage proteins and catalase enzyme in chickpea seeds (Cicer arietinum L.) in Gorgan University of Agricultural Science and Natural Resource seed laboratory at 2015. Experiment was in complementary randomized design arrangement with four replications. Treatments were 2 and 4 years natural storage and 1-5 days of accelerated ageing with control treatment. After treatments performance, traits such as germination percentage, germination rate, seedling dry weight, seed vigor index, soluble protein, protein carbonylation, protein electrophoretic pattern and catalase activity and their present in gel was investigated. The results showed that, with increasing of accelerated ageing level and storage duration, germination percentage and rate, seedling dry weight and seed vigor index were decreased. Soluble protein decreased and protein carbonylation decreased as ageing level increased that this procedure in4 and 5 days of accelerated ageing was higher than natural storage treatments. Results of SDS-PAGE pattern of chickpea seed proteins showed that there are not significant difference between accelerated ageing and control but in 4 years natural storage treatment deleted a light and 9.9 kDa protein band. This protein band was recovered after 4, 8 and 12 h imbibition of seeds. Catalase enzyme activity was higher in control treatment and increasing of accelerated ageing until 3 days catalase activity was increased then decreased.  Decreasing of catalase enzyme activity in 4 and 5 accelerated ageing treatments was higher than natural storage treatments. Native-PAGE results showed that there is one catalase activity band in chickpea. With increase of accelerated ageing and natural storage catalase band intensity was reduced that indicates the reduction of enzyme presence in the above treatments and supporting the enzyme activity in different ageing treatments. Results of the present study showed accelerated ageing and natural storage changed seed storage proteins quality and quantity and reduces catalase activity. These factors related to reduction of seed vigor and germination percentage and rate and seedling dry weight that this reduction in severe accelerated ageing was higher than natural storage.