اثر داروی کتوتیفن در مهار ورود انگل توکسوپلاسما گوندیی به داخل سلول‌های ماکروفاژ موش سوری

Background and purpose: Toxoplasma gondii is a protozoon with worldwide distribution. In spite of increasing information about its biology, treatment of toxoplasmosis is restricted to a few drugs and unfortunately using of each of drugs is associated with significant side effects in patients. It seems that investigation on alternative drugs is necessary. Materials and methods: In case group, concentrations of 1, 5 and 10 µg/ml of ketotifen were prepared and added to RPMI (Roswell Park Memorial Institute) medium containing peritoneal macrophages. After 60 minutes, incubation and adding Toxoplasma gondii tachyzoites to medium, the inhibitory rate of different concentrations of ketotifen on entrance of Toxoplasma tachyzoites into macrophages in 30 and 60 minutes were evaluated. Control group did not receive ketotifen. Results: The best efficacy of ketotifen in inhibition of entrance of Toxoplasma tachyzoites into macrophages was observed in 10 µg/ml and in 60 minutes (72.90 ± 1.70) (P < 0.05). Conclusion: The findings of this research show that ketotifen is a suitable drug for inhibiting the entrance of Toxoplasma gondii tachyzoites into nucleated cells in vitro.