مقایسه اثر مهارکنندگی و ضدباکتریایی عصاره‌ی آبی و اتانولی کرفس کوهی (Kelussia odoratissima)بر تعدادی از باکتری‌های بیماری‌زا در شرایط آزمایشگاهی

gte mso 9]>l>     M. Heidari Sureshjani[1], F. Tabatabaei Yazdi[2], A. Mortazavi[3], F. Shahidi3   Received: 20/05/2014 Sent for Revision: 09/07/2013 Received Revised Manuscript: 27/08/2014 Accepted: 09/09/2014   Background and Objective: Nowadays, the increasing use of antibiotics, or failure to comply the recommended dose by the patients, has led to the development of bacterial resistance to antibiotics. It has resulted in increasing the interest to use more efficient new anti-microbial compounds without toxicity, such as medicinal plants. The aim of this study was to compare the antibacterial activity of aqueous and ethanolic extracts of Kelussia odoratissima on some pathogenic bacteria. Materials and Methods: In this laboratory study, the antibacterial effect of aqueous and ethanolic extracts of Kelussia odoratissima leaves were investigated using pour plate and disk agar diffusion tests. Minimum Inhibitory Concentration (MIC( and Minimum Bactericidal Concentration (MBC) were also studied using the Dilution method. Analysis of results was performed using One-way analysis of variance (ANOVA). Results: MIC of aqueous and ethanolic extracts for Staphylococcus epidermidis and Streptococcus pyogenes was 32 and 16 mg/ml and for Pseudomonas aeruginosa was 64 and 32 mg/ml, respectively. MBC of aqueous and ethanolic extracts for Staphylococcus epidermidis and Streptococcus pyogenes was 64 and 32 mg/ml and for Pseudomonas aeruginosa was 128 and 64 mg/ml, respectively. The results of One-way analysis of variance showed, with increasing concentrations of ethanolic and aqueous extracts, inhibition zone was significantly increased. Conclusion: The results showed that the ethanolic extract of Kelussia odoratissima leaves had greater inhibitory effects on the strains studied in comparison with to aqueous extracts in vitro. Key words: Antimicrobial effect, Aqueous and ethanolic extracts, Kelussia odoratissima   Funding: This research was funded by Department of Food Sciences and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Ferdowsi University of Mashhad approved the study.   How to cite this article: Heidari Sureshjani M, Tabatabaei Yazdi F, Mortazavi A, Shahidi F. Comparison of the Inhibitory and Antibacterial Effect of Aqueous and Ethanolic Extract of Kelussia odoratissima on Some Pathogenic Bacteria "in vitro". J RafsanjanUniv Med Sci 2014 13(9): 775-884. [Farsi]   [1]- MSc Student of Food Sciences and Technology, Dept. of Food sciences and technology, Agriculture School, Ferdowsi University, Mashhad, Iran  (Corresponding Author): Tel: (0382) 3222612, Fax: (0382) 3224255, E-Mail: [email protected] [2]- Associate Prof., Dept. of Food sciences and technology, Agriculture School, Ferdowsi University, Mashhad, Iran [3]- Prof., Dept. of Food sciences and technology, Agriculture School, Ferdowsi University, Mashhad, Iran gte mso 9]>l> Normal 0 false false false EN-US X-NONE AR-SA     gte mso 9]>l>   /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0cm 5.4pt 0cm 5.4pt mso-para-margin:0cm mso-para-margin-bottom:.0001pt mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi}