Effect of Explant, Type and Concentration of Hormone on Callus Induction and Regeneration of two Iranian Sesame (Sesamum indicum L.) Cultivars

نویسندگان

چکیده مقاله:

Extended Abstract Introduction and Objective: Sesame is an ancient oilseed plant known for its medicinally important lignans and its high quality edible oil. ptimization of tissue culture in order to achieve high frequency regeneration is the first step for genetic transformation. the plant recalcitrant to plant tissue culture thus limiting the use of modern biotechnology for its genetic improvement. Therefore, sesame is one of the plants that need a suitable protocol for its Tissue culture and effective regeneration. Material and Methods: In this study, the rate of callus induction and regeneration three explants (cotyledon1, cotyledon2, hypocotyls) two sesame cultivars (Darab, Dashtestan) tested in different hormones including BAP in five levels (0, 4.5, 5.5, 6.5 and 7.5 mg/l), IAA in four levels (0, 0.5, 1 and 2 mg/l), Agno3 (5 mg/l). The shoots transferred to rooting media after regeneration. The experiment was conducted as a completely randomized design )CRD( with factorial arrangement and 3 replications. Results: According to the results, regeneration induction in hypocotyls explants and callus induction in cotyledon 1 and cotyledon 2 explants were not observed in any of the genotypes. Results of means comparison revealed best result for traits (fresh weight, dry weight, diameter callus and callus induction) In MS medium containing supplement of 5.5 mg/l BAP and 1 mg/l IAA, 6.5 mg/l BAP and 1 mg/l IAA cultivar Dashtestan, 5.5 and 6.5 mg/l BAP in 1 mg/l IAA cultivar Dashtestan, 5.5 mg/l BAP in 0.5 and 1 mg/l IAA cultivar Dashtestan. Conclusion: the highest rate of regeneration occurred in cotyledon1 explants with 6.5 mg / l BAP and 1 mg / l IAA. Finally, this medium is an efficient medium for the regeneration of these cultivars of sesame. All shoots formed roots after 15 days and Plantlets were transferred into pot with perlite and Cocopeat bed.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

effect of seed priming and irrigation regimes on yield,yield components and quality of safflowers cultivars

این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...

effect of listening strategy training on iranian efl learners listening comprehension and use of strategies

this study aimed to investigate the effects of listening strategy training on iranian efl learners listening comprehension and use of such strategies. this work, employing an experimental methodology, was conducted among 60 adult efl learners from a language institute in isfahan, iran, as participants. the participants, who were selected based on the results of a placement test, were assigned t...

15 صفحه اول

Floral Biology and pollination requirements of sesame (Sesamum indicum L.)

This study aimed to investigate the floral biology and pollination requirements of sesame (Sesamum indicum L.), cultivar CNPA G2. The study was carried out at Barbalha, State of Ceará, and consisted of two phases: floral biology and pollination requirements. Flowers were observed as for time of anthesis, lifespan and stigma receptivity, and were applied the following pollination treatments: ope...

متن کامل

the effects of planning on accuracy and complexity of iranian efl students’ written narrative task performance

this study compared the different effects of form-focused guided planning vs. meaning-focused guided planning on iranian pre-intermediate students’ task performance. the study lasted for three weeks and concentrated on eight english structures. forty five pre-intermediate iranian students were randomly assigned to three groups of guided planning focus-on-form group (gpfg), guided planning focus...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 14  شماره 41

صفحات  205- 214

تاریخ انتشار 2022-03

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

کلمات کلیدی برای این مقاله ارائه نشده است

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023