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Effect of Nigella sativa fixed oil on ethanol toxicity in rats

نویسندگان

  • Alireza Timcheh Hariri Medical Toxicology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Elahe Taghiabadi Department of Phamacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Hamed Pourbakhsh Food Control Laboratory, Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran
  • Hossein Hosseinzadeh Pharmaceutical Research Center, Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Khalil Abnous Pharmaceutical Research Center, Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Sayed Masoud Hosseini Department of Phamacodynamy and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

چکیده

Objective(s):This study was planned to appraise the protective effect of Nigella sativa fixed oil (NSO) against subchronic ethanol induced toxicity in rats. Materials and Methods: Studies were carried out on six groups of six animals each, including control (normal saline, gavage), ethanol (3 g/kg/day, gavage), NSO (0.125, 0.25 and 0.5 ml/Kg/day, IP) plus ethanol and NSO (0.5 ml/Kg/day, IP) groups. Treatments were continued for 4 weeks. Results: According to data, treatment with NSO attenuated ethanol-induced increased levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), as well as histopathological changes in liver and kidney tissues. Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats. Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney. Conclusion: This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

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منابع مشابه

Background: In this study, the protective effect of thymoquinone (TQ) and Nigella sativa seeds oil (NSO) was evaluated against oxidative damages induced by ethanol in rats. Methods: Animals were treated with ethanol 40% daily by gavages once a day for 4 weeks. NSO and TQ were injected intraperitoneal once a day for 4 weeks. The histopathological examination of liver, kidney, brain and heart was...

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