P-59: Low IVF Rate and Embryonic Development in Ciprofloxacin-Treated Male Mice
Background: In the last few years, a marked decrease in male fertility has been reported. Environmental factors were recently suspected for this problem. The misuse of medicines especially antibiotics are among environmental factors that can affect reproductive system. Ciprofloxacin is a synthetic antibacterial agent belonging to the family of fluoroquinolones with a very broad spectrum effect against microbial pathogens. The aim of this study was to investigate the adverse effects of ciprofloxacin on ivf rate and embryonic development in male mice. Materials and Methods: Adult NMRI mice were randomized and kept on standard diet pellets and water adlibitum.ciprofloxacin was suspended in carboxymethyl cellulose(CMC) and given orally in doses of 206,412mg/kg body weight once daily for 45 consecutive days.CMC was given concurrently to control animals.At the end of the study the animals were sacrificed humanely and sperm processing for in vitro fertilization was performed,then sperms was added to HTF+BSAmedium containing the oocytes.All of the HTF droplets were covered with mineral oil.The rate of fertilization,two cell embryos,blastocysts,arrested embryos and type of them was examined in period of 120 hours. Results: A significant decrease in fertilization rate,percentage of two cell embryos and blastocysts were observed in ciprofloxacin - treated groups compared to the control group.Moreover administration of ciprofloxacin caused significant increase in embryo arrest rate. Conclusion: The present study showed that administration of ciprofloxacin decreased in vitro fertilization rate and percentage of two cell embryos and blastocysts.
Background Oxidative stress induced by cyclophosphamide (CP), as a chemotherapeutic agent, causes vulnerability of sperm and declining the fertility capacity. Then in this study the effect of crocin (saffron extract) on amelioration of these side effects was investigated. MaterialsAndMethods In this study 24 mice were divided in three groups (n=8). Control group received normal saline (0.2 ml/d...متن کامل
The effect of ciprofloxacin on sperm DNA damage, fertility potential and early embryonic development in NMRI mice
Side effects of ciprofloxacin (CPFX), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. In the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm DNA damage using Aniline Blue and Acridine Orange technique, respectively. The fertility potential in male mice was also evaluated. NMRI male...متن کامل
Protective Effects of Wheat Germ Oil on the IVF, Oxidative Stress and In Vitro Zygote Development in Carbamazepine Treated Epileptic Male Mice
Abstract Background and Objectives: Epilepsy is a neurological disorder caused by abnormal electrical discharges from the brain. Carbamazepine is used to treat epilepsy. Over time, this drug may increase oxidative stress and reproductive abnormalities in patients with epilepsy. Wheat germ oil has the highest amount of vitamin E, among other vegetable oils. The aim of this study was to evaluate ...متن کامل
FTER parental exposure to mutagens and subsequent mating, adverse A genetic effects may manifest sequentially by preimplantation losses of fertilized ova and zygotes, early fetal deaths, and sterility and semisterility in F, progeny ( CATTANACH 1964; EPSTEIN et al. 1970a). Effects of ionizing irradiation of the male or of sperm in uitro on fertilization and subsequent development of the zygote ...متن کامل
P-125: Assessment The Effect of Equisetum Arvense Alcoholic Extract on IVF Rate, Embryo Quality and Development in STZ-Induced Diabetic Female Mice
Background: Diabetes mellitus is a common metabolic circumstance characterized by high blood glucose level and partial or absolute impairment of insulin secretion. Diabetes in females leads to sexual disorders such as loss of libido, low ovulation rate and disorder in oocyte maturation. Therefore the current study was designed to evaluate the protective effect of Equisetum arvense alcoholic ext...متن کامل
In order to evaluate the effect of O2 tension on the in vitro maturation (IVM) and fertilization (IVF) process, 11 replicates of OPU/IVP were performed using 1092 viable oocytes obtained from 48 Bos indicus embryo donors of commercial herds. Cumulus-oocyte complexes (COCs) from each donor were homogeneously and randomly distributed across two treatments. The IVM was performed in TCM-199 with ov...متن کامل
دوره 5 شماره Supplement Issue
تاریخ انتشار 2011-09-01
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