Specific Chicken Egg Yolk Antibodies against Enterotoxigenic Escherichia coli K99 in Serum and Egg Yolk of Immunized Laying Hens
Enterotoxigenic Escherichia coli K99 is one of the dominant pathogens associated with diarrhea of calves. Immunoglobulin Y (IgY), has been used as an inexpensive alternative to antibiotics for the prevention and therapy of several bacterial infections. The study aimed to prepare IgY antibodies against E. coli K99 and to investigate its in vitro effectiveness. E. coli K99 was grown in the tryptic soy broth, and the bacterial suspension was inactivated by formaldehyde. Thirty White Leghorn hens allocated to control and treatment groups. 1 mL of the prepared bacterial suspension or sterilized physiological serum emulsified with Freund’s complete adjuvant was injected at two weeks interval to the hens in treated or control group. The total IgY was purified with polyethylene glycol (PEG) 6000 from egg yolks (EY). Purified IgY fractions were processed for protein concentrations by Bradford assay. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze purified IgY fractions. An indirect enzyme-linked immunosorbent assay (ELISA) method was used to measure the specific IgY titers in serum and EY. The ability of non-specific and anti-E. coli K99 IgY antibody at 100 and 200 mg/mL concentration was evaluated by growth inhibition assay in vitro. According to the ELISA data, total IgY concentration in serum and EY of control was relatively constant, while increased in the treatment group (P<0.05). The level of binding activity of specific IgY in serum and EY increased in immunized hens (P<0.05). Specific IgY had the highest activity for bacterial inhibiting growth at the level of 200 mg/mL. These results suggested the prepared IgY antibodies could inhibit E. coli K99 in vitro growth.
Reflection of serum immunoglobulin isotypes in the egg yolk of laying hens immunized with enterotoxigenic Escherichia coli
Aim: The aim was to study the seroconversion and development of egg yolk immunoglobulins in adult laying White Leghorn hens immunized against an isolate of enterotoxigenic Escherichia coli (ETEC) bearing K91 and K88ac antigens, obtained from diarrheic piglet. Materials and Methods: Adult laying White Leghorn hens were immunized with inactivated enterotoxic E. coli strain isolated originally fro...متن کامل
Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in both humans and animals. The contaminated food and water are the most common vehicles for ETEC infection. The colonization factor antigen (CFA-1) is a fimbriae protein that promotes adherence of the ETEC strain to the epithelium of the small intestine of the host. In this study IgY proteins were produced against the CFA-1 of ETEC in imm...متن کامل
Comparison of Four Different Purification Methods for Isolation of Anti Echis carinatus Antivenom Antibodies from Immunized Chicken Egg Yolk
Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...متن کامل
Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets.
Passive protection of neonatal piglets against fatal enteric colibacillosis was achieved with powder preparations of specific antibodies against K88, K99, and 987P fimbrial adhesins of enterotoxigenic Escherichia coli. The antibody powders were obtained by spray drying the water-soluble protein fraction of egg yolks from immunized hens after the lipid components were precipitated with an aqueou...متن کامل
Monacolin K is a secondary metabolite of Monascus species and reduces cholesterol levels. This research focuses on the effect of adding red mold rice to hens' diet on cholesterol level in egg yolk and on cholesterol, triglyceride, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels in serum. Forty-eight Hy-line laying hens of 48 weeks of age were studied by dividing them in...متن کامل
Differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.
Cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized Leghorn laying hens and an enzyme-linked-immunosorbent assay. The effects of the four species on the hens were compared on various days postimmunization. Hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but on...متن کامل
دوره 10 شماره 1
صفحات 155- 161
تاریخ انتشار 2020-03-01
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