The intensity of electrophoretic bands containing egg non-vitellogenin derived proteins in relationship with embryo-larvae viability in common dentex (Dentex dentex)  a preliminary evaluation

نویسنده

چکیده مقاله:

Despite the reported effects of egg vitellogenin-derived proteins (VtgDP) and amino acids on embryo-larvae viability in common dentex the effects of non-vitellogenin-derived proteins (non-Vtg-DP) has yet to be determined. As an initial study, fertilized eggs (70 batches) were provided by natural spawning of broodfish in captivity. Viability parameters (VP) such as egg floating, hatching, and larval survival rate were calculated. Egg batches were categorized as low, medium, and high quality upon hatching rate. Based on the SDS-PAGE of egg crude extracts 63 protein bands (16.7 to 131.4 kDa) were identified. The intensity of bands on gel (densitometry; <200 to 877 μg mL-1), and their biochemical nature (specific staining) was determined. After subjecting the bands to a screening process, 11 bands were considered as non-vtgDP-contained bands. The highest intensity of the bands was observed in the gels belonging to high quality eggs. The intensity of 9 bands of 11 (individually or in combination with other protein bands) was significantly (p<0.001) and positively correlated to VPs via a series of simple and multiple regression models. In brief, the preliminary results revealed the importance of the class of egg proteins and their effects (individual and combined) on the viability of common dentex larvae and indicated their probable potential use to predict egg quality in teleosts. It also showed that it is worth to conduct a deeper study for more detailed characterization of the protein content of the selected bands and to evaluate the mechanism of their effects on larval success

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver.

The aim of this work was to evaluate the effects of prolonged starvation and refeeding on antioxidant status and some metabolic-related parameters in common dentex (Dentex dentex) liver. Fish deprived of food for 5 weeks showed a significant increase in lipid peroxidation, measured as malondialdehyde (MDA) levels. The activity of the antioxidative enzymes superoxide dismutase (SOD), catalase, a...

متن کامل

Disinfection of Gilthead Sea Bream (Sparus aurata), Red Porgy (Pagrus pagrus), and Common Dentex (Dentex dentex) Eggs from Sparidae with Different Disinfectants

The aim of this study is to determine suitable conditions for egg disinfection of gilthead sea bream (Sparus aurata), red porgy (Pagrus pagrus), and common dentex (Dentex dentex) with glutaraldehyde, hydrogen peroxide and iodine. The eggs were disinfected with 200 ppm glutaraldehyde (T= 2, 4, 8, 16 min), 300 ppm hydrogen peroxide and iodine (T= 5, 10, 15, 20 min) at four different contact times...

متن کامل

Secondary structure of chorion proteins of the teleostean fish Dentex dentex by ATR FT-IR and FT-Raman spectroscopy.

FT-Raman spectroscopy and ATR-IR spectroscopy were applied to study the secondary structure of the eggshell (chorion) proteins of the teleostean fish Dentex dentex. Raman and IR spectra clearly indicate an abundance of antiparallel beta-pleated sheet conformation in chorion proteins. This finding is further supported by analysis of the vibrational data by regression techniques and deconvolution...

متن کامل

Dietary intake of probiotics and maslinic acid in juvenile dentex (Dentex dentex L.): effects on growth performance, survival and liver proteolytic activities

Two feeding trials were carried out to evaluate the efficiency of probiotics and maslinic acid, on growth and survival of juvenile dentex; liver proteolytic activities were also investigated in the second trial. For experiment 1, triplicate groups were fed six diets with two probiotics (Bacillus toyoi, T, and B. cereus, E) at increasing levels (0.5, 1 and 2 g kg diet) and a control diet. Growth...

متن کامل

Neurotransmitters, neuromodulators, and neurotrophin receptors in the gut of pantex, a hybrid sparid fish (Pagrus major x Dentex dentex). Localizations in the enteric nervous and endocrine systems.

The gut of Pantex, a sparid hybrid fish (Pagrus major x Dentex dentex) with a great potential importance for the Italian aquaculture, was histochemically and immunohistochemically investigated in order to evidence components of the intramural nervous and diffuse endocrine systems. The general structural aspects of the intramural nervous system were shown by the Nissl-thionin staining. As in mos...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 9  شماره 4

صفحات  41- 50

تاریخ انتشار 2021-12

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

کلمات کلیدی برای این مقاله ارائه نشده است

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023