sex determination in pistacia as a dioecious plant is economically desirable. identification of the male and female plants has a great value for pistacia yield production. in the present study, leaf samples from four female plants from cultivars of pistacia including akbary, akbar aghaei, fandoghi, and kalleh ghochi as well as male plants were used for rapd pcr amplification. among 32 primers w...

in recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of the environmental contaminants. in this study, the root length and dna band pattern of root tips in maize (zea mays l.) seedlings were exposed at different concentration of cadmium pollutant (40 and 80 mg l−1). the results indicated that the root length of ma...

rhizomania is the most important disease of sugar beet in iran and some other parts of the world, and plays an essential role in decreasing sugar yield in fields. the best approach to control this disease is to use resistant varieties. tagging the resistance genes in breeding programs, by molecular markers is necessary. in this study, some breeding populations and commercial varieties of sugar ...

The RAPD method (Random Amplified Polymorphic DNA) was used for identifying and mapping new molecular markers in pea. RAPD analysis of various cultivars and lines of pea was carried out using 10-mer random primers. The presence of multiple polymorphism between cultivars and lines was revealed; at least one fragment for any given primer was present in the DNA of one form of pea and absent in the...

BACKGROUND Pseudomonas aeruginosa is one of the important causes of nosocomial infections that easily gains resistance to many antibiotics. This opportunistic pathogen is a major health hazard particularly in immunodeficient patients, patients in intensive care units (ICU) and burn units with life threatening outcome. The bacterium may be originated from different or common sources, and compris...

  Background: Garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. The analysis of genetic diversity plays an important role in breeding programs. The RAPD technique could be very effective in detecting genetic variation in garlic.  Objective: The objective of the present work was to detect molecular polymorphism among Iranian garli...

Research to determine patterns of genetic differentiation among and within field populations of Crom.um quercuum f. sp.firsifome using RAPD markers is currently underway in the molecular genetics laboratory at the Southern Institute of Forest Genetics. Fungal tissue was collected as a drop of spermatia or scrapings of a discrete hymenium fiom a single gall on each tree sampled at a location. Co...

A simple and efficient protocol for isolating genomic DNA from fresh and dry leaves of Vigna aconitifolia and V. trilobata was developed. It involves a modified CTAB procedure using 3% CTAB, 4% -mercaptoethanol, 2 M NaCl and 5% PVP. The extraction was carried out at 70°C. A slight increase in the concentrations of these chemical components and temperature helped in the removal of secondary meta...

BACKGROUND & OBJECTIVES Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the ra...