Background: Phenol is an aromatic pollutant in industrial wastes that in combination with salts is highly toxic for all forms of life. Phenol elimination is the foremost challenge to meet the goal of pollutant-free environment. Objective: The present study was carried out to isolate phenol degrading bacteria which can degrade phenol under saline conditions and...

Objective(s):Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. GroEL antigen increases Brucella survival and is one of the major antigens that stimulates the immune system. Hence, the objective of the present study was cloning and bioinformatics analysis of GroEL gene. Materials and Methods: The full-length open reading frame of this gene was...

Phenylketonuria (PKU) is one of the most common known inherited metabolic diseases. The present study aimed to investigate the status of molecular defects in phenylalanine hydroxylase (PAH) gene in western Iranian PKU patients (predominantly from Kermanshah, Hamadan, and Lorestan provinces) during 2014-2016. Additionally, the results were compared with similar studies in Iran. Nucleotide sequen...

Tomato yellow leaf curl virus (TYLCV) is a supreme pathogen in tropical and subtropical areas. During 2014-2015, a total of 393 tomato samples showing Tomato yellow leaf curl disease (TYLCD) symptoms were collected from six different provinces of Iraq. In serological assays, 55 out of 393 samples (14%) reacted positively with TYLCV-specific antibodies .The presence of TYLCV was verified in 21 (...

In this study, molecular characterization of Echinococcus granulosus sample obtained from water buffalo originating from southwest of Iran was performed using comparative sequence analysis of cox1 mitochondrial gene. DNA was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in Khuzestan province. Molecular and phylog...

Background: Several methods have been developed for detection of sequence variation in genes and each has its advantages and disadvantages. A disadvantage of them is that the simpler, cost-effective methods are commonly perceived as being less sensitive in their detection of sequence variation, whereas those with proven sensitivity have a requirement for complex or expensive laboratory equipmen...

background: cholesterol oxidase (cho) has various clinical and industrial applications. recently, microbial cho have received a great attention for their wide usage in medicine. here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for cho production and biochemical characterizations of produced cho enzyme were described. finally, cho gene was cloned int...