Objective: L-asparaginase is an enzyme of industrial as well as therapeutic importance. The capabilities of bioprocess modeling of L-Asparaginase activity produced from Aspergillus niger by solid state fermentation (SSF) were explored here. Methods: Regression modeling (RM) and Artificial Neural Network (ANN) techniques were applied on input process parameter, which includes solid substrate, te...

T-Acute Lymphoblastic Leukemia (T-ALL) remains a subgroup of pediatric ALL, with a lower response to standard chemotherapy. Some recent studies established the fundamental role of epigenetic aberrations such as DNA hypermethylation, to influence patients' outcome and response to chemotherapy. Moreover, L-asparaginase is an important chemotherapeutic agent for treatment of ALL and resistance to ...

Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenoty...

We prospectively studied the incidence and clinical course of hypertriglyceridemia and hypercholesterolemia during very prolonged use of asparaginase in relation to levels of asparaginase activity in children with acute lymphoblastic leukemia. We also evaluated the incidence of pancreatitis, thrombosis, hyperammonemia and central neurotoxicity and their association with asparaginase activity le...

Backgroung and Aims: L-Asparaginase II is a cornerstone of treatment protocols for acute lymphoblastic leukemia. Only asparaginase II obtained from E. coli K12 and Erwinia chrysanthemi have been used in human as therapeutic drug. The therapeutic effects of asparaginase II from E. coli K12 and Erwinia chrysanthemi is accompanied by side effects. It is desirable to search for other asparaginase I...

The antitumor enzyme asparaginase, which targets essential amino acid L-asparagine and catalyzes it to L-aspartic acid and ammonia, has been used for years in the treatment of acute lymphoblastic leukemia (ALL), subtypes of myeloid leukemia and T-cell lymphomas, whereas the anti-chronic myeloid leukemia (CML) effect of asparaginase and its underlying mechanism has not been completely elucidated...

Survival times of C57BL/6J X DBA/2J Fj mice, inoculated with LI210 or LSI78Y ascites tumor cells, were monitored following treatment with cytosine arabinoside, L-asparaginase, and 6-azauridine, alone and in combination. Each tumor line was sensitive to cytosine arabinoside. L-Asparaginase produced cures (60-day survivors) in 20 to 30% of L5178Y hosts but was totally ineffective against L1210. 6...

OBJECTIVES Combining vorinostat, L-asparaginase, and doxorubicin (Dox) led to improved response rates in the treatment of lymphoid tumors. However, deep-vein thrombosis has been noted as one of the most serious side effects with these drugs, and how these regimens cause deep-vein thrombosis is unclear. METHODS We investigated the procoagulant effects of vorinostat, L-asparaginase, and doxorub...

The aim of this investigation was to compare the efficacy of the permeabilization methods of microwave irradiation and potassium phosphate (KPi)-hexane solvent system in isolating the periplasmic enzyme, L-asparaginase II. Previous studies have shown that both methods enable the isolation of periplasmic proteins in Gram-negative bacteria. Periplasmic, cytoplasmic and membrane fractions of Esche...