نتایج جستجو برای: nested pcr the results of pcr

تعداد نتایج: 22835081  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده منابع طبیعی و علوم دریایی 1388

this research was conducted in two protect and destroy region in the middle zagros, in illam province. in order to identification of ecological species group and evaluate density of regeneration, effect of many factors such as protection, vegetation, physiographic factors, physical and chemical properties of soil in study locations were studied. to achieve these purpose number of 54 plots using...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان تهران - دانشکده زبانهای خارجی 1391

as polysemy is encountered frequently in english as foreign language. fl learners’ ability to disambiguate polysemous verbs becomes critical to their comprehension in the target language. this thesis, accordingly, investigated how iranian efl learners achieved comprehension of english polysemous verbs by using three different types of cues: (1) elaborated context, (2) semantic frames, and (...

پایان نامه :دانشگاه تربیت معلم - تهران - دانشکده ادبیات و علوم انسانی 1392

in this study, the relationship between teachers’ creative and critical thinking skills and their reflectivity in teaching was investigated. to this end, three questions were raised. these questions were intended to determine whether there was a significant relationship between teachers’ creative thinking skills and their reflectivity in teaching; whether there was a significant relationship be...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی دکتر علی شریعتی 1391

this ethnographic case study research was carried out in a private school setting in the context of iran. the research tried to explore the analysis and identity construction of a group of learners and teachers along with the content analysis of books on the basis of four types of commodified, political, national and narrative identities. how english language learners and teachers in an informa...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه علوم بهزیستی و توانبخشی - دانشکده توانبخشی 1393

abstract objectives gradual increase length and complexity of utterance (gilcu) therapy method is a form of operant conditioning. this type of treatment is very precise and controlled that is done in 54 steps in 3 speech situations consisted of monologue, reading and conversation. this study aimed to examine the effects of gilcu treatment method on reduction of speech dysfluency of school-age...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه پیام نور - دانشگاه پیام نور استان همدان - دانشکده ادبیات و علوم انسانی 1390

the present research has been deducted from a provincial research project which aims at determining the relationship of variables of variables of occupational self-concept, intelligence beliefs and metacognitive with entrepreneurship among the students of payame noor university of kurdistan. the volume of the samples was 1080 students (576 female and 504 male students). the research methodology...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - دانشکده دامپزشکی 1391

after test and review results and analysis of farm data, the following results were obtained. the review examined farms was done and the results obtained in this study was to determine the effectiveness of vaccines hatchpak avinew (frozen) and hatchpak ib h120 with spray in broilers a day in factories incubation and at least,they can be as vaccines avinew and bioral h120 ability to create saf...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد گرمسار - دانشکده ادبیات و زبانهای خارجی 1391

this study was an attempt to investigate the effect of teaching meta cognitive strategies on iranian intermediate efl students speaking proficiency. in this study the researcher has employed metacognitive strategies taken from brown (2000) to teach speaking strategies to number of participants. the participants were intermediate students of shokouh language institute; they were divided into an ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه زنجان - دانشکده علوم انسانی 1389

abstract this study evaluated and compared medical terminology and english for the students of medicine (ii) as two representatives of the textbooks available on the iranian market for teaching english to the students of medicine. this research was performed on the basis of a teacher’s and a number of students’ attitudes and the students’ needs analysis for two reasons: first, to investigate...

Journal: :تحقیقات دامپزشکی 0
سید علی قرشی گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری مرتضی دلیری جوپاری گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری دینا مرشدی گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری ترانه حاجیان گروه میکروبیولوژی، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری محسن لطفی موسسه تحقیقات واکسن و سرم سازی رازی کرج

in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

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